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<p>This study aimed to evaluate gold nanoparticles (GNPs) and photobiomodulation (PBM), associated with antibothropic serum (AS), to treat a muscle lesion induced by <i>Bothrops jararaca</i> venom. Methods: 108 Swiss male mice were used, divided into nine groups (<i>n</i> = 12) with different combinations of treatments. Animals were inoculated with 250&#xa0;µg of <i>B. jararaca</i> venom at the right gastrocnemius muscle. The treatment started 12&#xa0;h after the venom injection and occurred daily for 7&#xa0;days. AS was administered as recommended by the Ministry of Health. Low-power aluminum gallium indium phosphide (AlGaInP) with continuous emission (wavelength 660&#xa0;nm; average power 30 mW ± 20%; beam size of 0.06310 cm<sup>2</sup>; and dose of 2&#xa0;J for ± 1&#xa0;min per point). Irradiation was applied to five distinct areas surrounding the wound, at a perpendicular angle to the skin. The groups treated with GNPs received treatment through the application of 1&#xa0;mL of 20&#xa0;nm GNPs (30&#xa0;mg/L). Muscle, heart, and kidneys were removed for histological and biochemical analysis. Results: GNPs can improve the results achieved by PBM and the standard treatment, presenting significant benefits in histological parameters, such as the increase of new blood vessels, fibroblasts, collagen, and a reduction of inflammatory infiltrate. Conclusion: GNPs can be considered an option to accelerate the recovery of <i>Bothrops</i> lesions.</p> Graphical Abstract <p></p>

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A combination of gold nanoparticles and laser photobiomodulation to boost antioxidant defenses in the recovery of muscle injuries caused by Bothrops jararaca venom

  • 期刊论文

  • 发表日期：

    2025年1月29日

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  • Mírian Ívens Fagundes,
  • Nathalia Coral Galvani,
  • Camille M. Generoso,
  • Ellen De-Pieri,
  • Rahisa Scussel,
  • Bruna B. Fernandes,
  • Igor Ramos Lima,
  • Jéssica S. Abel,
  • Maria Eduarda A. B. Corrêa,
  • Jonathann C. Possato,
  • Paulo E. Feuser,
  • Thiago A. M. de Andrade,
  • Carlos Chávez-Olortegui,
  • Paulo Cesar Lock Silveira,
  • Ricardo Andrez Machado-de-Ávila

摘要

This study aimed to evaluate gold nanoparticles (GNPs) and photobiomodulation (PBM), associated with antibothropic serum (AS), to treat a muscle lesion induced by Bothrops jararaca venom. Methods: 108 Swiss male mice were used, divided into nine groups (n = 12) with different combinations of treatments. Animals were inoculated with 250 µg of B. jararaca venom at the right gastrocnemius muscle. The treatment started 12 h after the venom injection and occurred daily for 7 days. AS was administered as recommended by the Ministry of Health. Low-power aluminum gallium indium phosphide (AlGaInP) with continuous emission (wavelength 660 nm; average power 30 mW ± 20%; beam size of 0.06310 cm2; and dose of 2 J for ± 1 min per point). Irradiation was applied to five distinct areas surrounding the wound, at a perpendicular angle to the skin. The groups treated with GNPs received treatment through the application of 1 mL of 20 nm GNPs (30 mg/L). Muscle, heart, and kidneys were removed for histological and biochemical analysis. Results: GNPs can improve the results achieved by PBM and the standard treatment, presenting significant benefits in histological parameters, such as the increase of new blood vessels, fibroblasts, collagen, and a reduction of inflammatory infiltrate. Conclusion: GNPs can be considered an option to accelerate the recovery of Bothrops lesions.

Graphical Abstract