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<p>Bacteriophages are promising bactericidal agents with potential for infection control, but purification methods and long-term storage can compromise their stability. Viral particle aggregation is a known cause of viability loss and may be reduced with surfactants such as Tween 20. This study evaluated the relationship between aggregation and infectivity loss in MS2 and PhiX174 purified by chloroform clarification and PEG concentration, with or without Tween 20, and by Triton X-100, over 55 days. Infectivity and aggregation varied by purification method, but no direct correlation emerged. MS2 and PhiX174 responded differently, indicating that stability protocols must be virus-specific.</p>

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Purification strategies and impact of surfactant on bacteriophage stability and aggregation

  • 期刊论文

  • 发表日期：

    2025年12月26日

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  • Estêvão Brasiliense de Souza,
  • Miguel Abreu de Oliveira,
  • Helena Yurevna Caio,
  • Aguinaldo Roberto Pinto,
  • Gislaine Fongaro

摘要

Bacteriophages are promising bactericidal agents with potential for infection control, but purification methods and long-term storage can compromise their stability. Viral particle aggregation is a known cause of viability loss and may be reduced with surfactants such as Tween 20. This study evaluated the relationship between aggregation and infectivity loss in MS2 and PhiX174 purified by chloroform clarification and PEG concentration, with or without Tween 20, and by Triton X-100, over 55 days. Infectivity and aggregation varied by purification method, but no direct correlation emerged. MS2 and PhiX174 responded differently, indicating that stability protocols must be virus-specific.