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Background <p>Phages are potent alternatives to antibiotics due to their lytic activity and specificity. We isolated and characterized a specific bacteriophage effective against resistant strains of <i>P. aeruginosa</i> and their biofilms.</p> Methods <p>Samples were collected from hospital sewage. The morphological and life-cycle characteristics, pH and thermal stability, and host range of the isolated phage were assessed. Additionally, the sensitivity of 42 P. aeruginosa strains and their biofilms was evaluated.</p> Results <p>Morphological analysis showed that vB-pae-PA01 resembled Podophages. Sensitivity assays revealed that vB-pae-PA01 lysed 57% of the strains. vB-pae-PA01 exhibited strong thermal stability. However, highly acidic and basic conditions reduced its lytic activity. The one-step growth curve revealed a latent period of 10&#xa0;min and a burst size of 105 PFU per cell.</p> Conclusion <p>We isolated the lytic bacteriophage vB-pae-PA01, demonstrating potent lytic activity against <i>P. aeruginosa</i> strains. These properties suggest that vB-pae-PA01 could be a potential candidate to use against antibiotic-resistant <i>P. aeruginosa</i>.</p>

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Isolation and characterization of a lytic phage and its efficacy against multi-drug resistant strains of pseudomonas aeruginosa

  • 期刊论文

  • 发表日期：

    2025年10月14日

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  • Mohammad Sarmast,
  • Hadi Peeri-Dogaheh,
  • Chiman Karami,
  • Farzad Khademi

摘要

Background

Phages are potent alternatives to antibiotics due to their lytic activity and specificity. We isolated and characterized a specific bacteriophage effective against resistant strains of P. aeruginosa and their biofilms.

Methods

Samples were collected from hospital sewage. The morphological and life-cycle characteristics, pH and thermal stability, and host range of the isolated phage were assessed. Additionally, the sensitivity of 42 P. aeruginosa strains and their biofilms was evaluated.

Results

Morphological analysis showed that vB-pae-PA01 resembled Podophages. Sensitivity assays revealed that vB-pae-PA01 lysed 57% of the strains. vB-pae-PA01 exhibited strong thermal stability. However, highly acidic and basic conditions reduced its lytic activity. The one-step growth curve revealed a latent period of 10 min and a burst size of 105 PFU per cell.

Conclusion

We isolated the lytic bacteriophage vB-pae-PA01, demonstrating potent lytic activity against P. aeruginosa strains. These properties suggest that vB-pae-PA01 could be a potential candidate to use against antibiotic-resistant P. aeruginosa.