Scholarlink

<p>Detection of <i>Chlamydia trachomatis</i> (CT) and <i>Neisseria gonorrhoeae</i> (NG) in cryopreserved semen is crucial for screening sperm donors. The evaluation of the limit of detection (LOD) of the APTIMA Combo 2™ kit (Panther, Hologic) was performed on cryopreserved semen samples spiked with CT and NG at concentrations ranging from 1 to 106 IFU/mL or CFU/mL, respectively. The LOD was 102 IFU/mL for CT and 10 CFU/mL for NG in single infection or coinfection. An inhibitory effect of semen on amplification was highlighted. This study confirmed the performance of the APTIMA Combo 2™ kit for screening cryopreserved sperm samples before donation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evaluation of the APTIMA Combo 2™ kit for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in frozen semen

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月30日

查看全文
  • Thibaut Lutz,
  • Robin Chautard,
  • Maxime Lafontaine,
  • Merve Genc,
  • Arabella Touati,
  • Sabine Pereyre,
  • Olivia Peuchant,
  • Cécile Bébéar,
  • Fabien Garnier,
  • Sébastien Hantz

摘要

Detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) in cryopreserved semen is crucial for screening sperm donors. The evaluation of the limit of detection (LOD) of the APTIMA Combo 2™ kit (Panther, Hologic) was performed on cryopreserved semen samples spiked with CT and NG at concentrations ranging from 1 to 106 IFU/mL or CFU/mL, respectively. The LOD was 102 IFU/mL for CT and 10 CFU/mL for NG in single infection or coinfection. An inhibitory effect of semen on amplification was highlighted. This study confirmed the performance of the APTIMA Combo 2™ kit for screening cryopreserved sperm samples before donation.