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Objectives <p>This is the first study conducted in southern Spain to determine i) the population structure (PS) of carbapenem-resistant (CR) <i>Acinetobacter baumannii</i> isolates by multilocus sequencing typing (MLST) and core genome MLST (cgMLST) and ii) the association between the sequence type ST and the <i>bla</i><sub>OXA-51</sub> variant, capsule polysaccharide locus (KL) and lipooligosaccharide outer core locus (OCL) types.</p> Methods <p>Of 336 isolates submitted to the Andalusian reference laboratory (PIRASOA; December 2017–2020), 73 were subjected to WGS (MiSeq). The following analyses were performed: bacterial identification (ribosomal MLST), carbapenemase gene detection (Resfinder 4.0), PS delineation (MLST by MLSTfinder 2.0 and cgMLST by Ridom SeqSphere<sup>+</sup>), and KL types and OCL types (Kaptive tool).</p> Results <p>The carbapenemases detected were <i>bla</i><sub>OXA-23</sub> (n = 41), <i>bla</i><sub>OXA-58</sub> (n = 26), <i>bla</i><sub>OXA-24</sub> (n = 5), <i>bla</i><sub>OXA-72</sub> (n = 1) and <i>bla</i><sub>NDM-1</sub> (n = 2). The PS revealed one major ST2 clone (n = 54) and seven minor ST clones by MLST, and 41 lineages by cgMLST<sub>.</sub> Thirty-five lineages were detected only in a single hospital whereas five lineages were observed in several hospitals and provinces. <i>bla</i><sub>OXA-66</sub> was the most frequent <i>bla</i><sub>OXA-51</sub> variant and was mainly associated with the ST2 clone. Eleven KL types and 3 OCL types were assigned, with KL2 (n = 27), KL7 (n = 16) and OCL1 being the most frequent.</p> Conclusions <p>The PS of CR <i>A. baumannii</i> in Andalusia is characterized by a dominant ST2/<i>bla</i><sub>OXA-23</sub> clone and several lineages, showing local spread of lineages in most hospitals, and intercenter or interregional spread of a few lineages. Single-locus <i>bla</i><sub>OXA-51-like</sub> typing and KL typing may be useful as complementary preliminary typing tool.</p>

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Regional distribution of carbapenemase-producing Acinetobacter baumannii isolates in southern Spain (Andalusia)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月17日

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  • Felipe Fernández-Cuenca,
  • Salud Rodríguez-Pallares,
  • Lorena López-Cerero,
  • José Gutiérrez-Fernández,
  • María Fe Bautista,
  • Juan Antonio Sánchez Gómez,
  • Waldo Sánchez-Yebra Romera,
  • Mercedes Delgado,
  • Esther Recacha,
  • Alvaro Pascual

摘要

Objectives

This is the first study conducted in southern Spain to determine i) the population structure (PS) of carbapenem-resistant (CR) Acinetobacter baumannii isolates by multilocus sequencing typing (MLST) and core genome MLST (cgMLST) and ii) the association between the sequence type ST and the blaOXA-51 variant, capsule polysaccharide locus (KL) and lipooligosaccharide outer core locus (OCL) types.

Methods

Of 336 isolates submitted to the Andalusian reference laboratory (PIRASOA; December 2017–2020), 73 were subjected to WGS (MiSeq). The following analyses were performed: bacterial identification (ribosomal MLST), carbapenemase gene detection (Resfinder 4.0), PS delineation (MLST by MLSTfinder 2.0 and cgMLST by Ridom SeqSphere+), and KL types and OCL types (Kaptive tool).

Results

The carbapenemases detected were blaOXA-23 (n = 41), blaOXA-58 (n = 26), blaOXA-24 (n = 5), blaOXA-72 (n = 1) and blaNDM-1 (n = 2). The PS revealed one major ST2 clone (n = 54) and seven minor ST clones by MLST, and 41 lineages by cgMLST. Thirty-five lineages were detected only in a single hospital whereas five lineages were observed in several hospitals and provinces. blaOXA-66 was the most frequent blaOXA-51 variant and was mainly associated with the ST2 clone. Eleven KL types and 3 OCL types were assigned, with KL2 (n = 27), KL7 (n = 16) and OCL1 being the most frequent.

Conclusions

The PS of CR A. baumannii in Andalusia is characterized by a dominant ST2/blaOXA-23 clone and several lineages, showing local spread of lineages in most hospitals, and intercenter or interregional spread of a few lineages. Single-locus blaOXA-51-like typing and KL typing may be useful as complementary preliminary typing tool.