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<p>This study evaluated the anti-hypoxic activity of Biyang floral mushroom polyphenols (BFMPs) extracted using deep eutectic solvent (DES)-based ultrasound-assisted and ethanol methods. <i>In vitro</i>, BFMPs-DES (200 μg/mL) increased the viability of CoCl<sub>2</sub>-induced PC12 cells by 73.40% vs. the hypoxia group. <i>In vivo</i>, BFMPs-DES (200 mg/kg) prolonged the survival duration of mice under normobaric hypoxia by 54.24% vs. the hypoxia group. Chromatography analysis revealed a higher relative abundance of propyl gallate (PG, 22.58 ng/mg) in BFMPs-DES. PG pretreatment effectively increased cell viability, elevated GSH level, and reduced MDA production and LDH activity in CoCl<sub>2</sub>-induced PC12 cells. Integrated network pharmacology, bioinformatics, and immunohistochemistry validation indicated that PG pretreatment could regulate effectively the protein expression level of HIF-1α, EPO, and VEGF, thereby ameliorating hypoxia injury. These findings suggest that BFMPs-DES possess notable anti-hypoxic properties, with PG as the pivotal bioactive compound. This work expands the application potential of Biyang floral mushrooms against hypoxia.</p>

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Anti-hypoxic activity of Biyang floral mushroom polyphenols: deep eutectic solvent-based ultrasound-assisted extraction versus ethanol extraction

  • 期刊论文

  • 发表日期：

    2026年7月11日

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  • Yingying Zhao,
  • Chengyuan Xie,
  • Yuqing Wang,
  • Ting Wang,
  • Juanjuan Yi,
  • Jike Lu

摘要

This study evaluated the anti-hypoxic activity of Biyang floral mushroom polyphenols (BFMPs) extracted using deep eutectic solvent (DES)-based ultrasound-assisted and ethanol methods. In vitro, BFMPs-DES (200 μg/mL) increased the viability of CoCl2-induced PC12 cells by 73.40% vs. the hypoxia group. In vivo, BFMPs-DES (200 mg/kg) prolonged the survival duration of mice under normobaric hypoxia by 54.24% vs. the hypoxia group. Chromatography analysis revealed a higher relative abundance of propyl gallate (PG, 22.58 ng/mg) in BFMPs-DES. PG pretreatment effectively increased cell viability, elevated GSH level, and reduced MDA production and LDH activity in CoCl2-induced PC12 cells. Integrated network pharmacology, bioinformatics, and immunohistochemistry validation indicated that PG pretreatment could regulate effectively the protein expression level of HIF-1α, EPO, and VEGF, thereby ameliorating hypoxia injury. These findings suggest that BFMPs-DES possess notable anti-hypoxic properties, with PG as the pivotal bioactive compound. This work expands the application potential of Biyang floral mushrooms against hypoxia.