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<p>This study investigated the effects of freeze–thaw treatment on survival and stress responses in single-species biofilms of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> and their dual-species biofilm, designated as LM, PA, and LP, respectively. Biofilms were formed on stainless steel and subjected to different freezing and thawing conditions. Smaller freeze–thaw temperature shifts resulted in higher viable cell counts of LM and PA, reaching 4.79 ± 0.22 and 7.67 ± 0.17 log CFU/cm<sup>2</sup>, respectively. In contrast, LM, which showed the lowest extracellular polymeric substances (EPS) production, had the lowest viable count under the largest temperature shift (4.01 ± 0.17 log CFU/cm<sup>2</sup>). This survival pattern was accompanied by greater membrane damage and membrane potential changes under larger temperature shifts. LP produced 9.7- and 2.4-fold higher EPS component levels than LM and PA, respectively, and maintained structural stability and <i>L. monocytogenes</i> viability, suggesting potential protection by interspecies interactions and the biofilm matrix.</p>

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Survival and stress responses of Listeria monocytogenes and Pseudomonas aeruginosa in single- and dual-species biofilms on stainless steel under freeze–thaw conditions

  • 期刊论文

  • 发表日期：

    2026年6月19日

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  • Yoon-Mi Ji,
  • Se-Wook Oh

摘要

This study investigated the effects of freeze–thaw treatment on survival and stress responses in single-species biofilms of Listeria monocytogenes and Pseudomonas aeruginosa and their dual-species biofilm, designated as LM, PA, and LP, respectively. Biofilms were formed on stainless steel and subjected to different freezing and thawing conditions. Smaller freeze–thaw temperature shifts resulted in higher viable cell counts of LM and PA, reaching 4.79 ± 0.22 and 7.67 ± 0.17 log CFU/cm2, respectively. In contrast, LM, which showed the lowest extracellular polymeric substances (EPS) production, had the lowest viable count under the largest temperature shift (4.01 ± 0.17 log CFU/cm2). This survival pattern was accompanied by greater membrane damage and membrane potential changes under larger temperature shifts. LP produced 9.7- and 2.4-fold higher EPS component levels than LM and PA, respectively, and maintained structural stability and L. monocytogenes viability, suggesting potential protection by interspecies interactions and the biofilm matrix.