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<p>The purpose of this work was to optimise the hot water extraction process utilising response surface methodology with Box–Behnken design modelling of <i>Pleurotus djamor</i> polysaccharides (PDP). The ideal parameters of extraction were a ratio of solvent-to-solid of 10.15&#xa0;mL/g, 1.65&#xa0;h of time, and 82.08&#xa0;°C of temperature. The PDP extraction yield of 6.09% was obtained by hot water extraction. According to a chemical component analysis, PDP included 69.6% total carbohydrates, 6.86% protein, and 6.17% uronic acid. PDP demonstrated strong in vitro antioxidant scavenging action for OH<Emphasis FontCategory="NonProportional">˙</Emphasis>, Cu<sup>2+</sup>, Fe<sup>3+</sup>, β-carotene bleaching inhibition, and chelating ability. The PDP inhibited 53.09% of murine fibroblast (L929) and 57.58% of murine metastatic melanoma cells (B16–F10) at a concentration of 100&#xa0;μg/mL and possesses an immunomodulatory effect by enhancing macrophage phagocytosis. These results offer a comprehensive understanding of the efficient extraction of PDP and their importance in considering their potential applications.</p>

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Polysaccharide from Pleurotus djamor: extraction optimization with response surface methodology, preliminary structure, antioxidant, immunomodulatory and antitumor potentials

  • 期刊论文

  • 发表日期：

    2026年1月13日

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  • Sudha Govindan,
  • Jessy Durairaj,
  • Gayathri Rajendran,
  • Himabindu Padinjarathil,
  • Prasanna Ramani

摘要

The purpose of this work was to optimise the hot water extraction process utilising response surface methodology with Box–Behnken design modelling of Pleurotus djamor polysaccharides (PDP). The ideal parameters of extraction were a ratio of solvent-to-solid of 10.15 mL/g, 1.65 h of time, and 82.08 °C of temperature. The PDP extraction yield of 6.09% was obtained by hot water extraction. According to a chemical component analysis, PDP included 69.6% total carbohydrates, 6.86% protein, and 6.17% uronic acid. PDP demonstrated strong in vitro antioxidant scavenging action for OH˙, Cu2+, Fe3+, β-carotene bleaching inhibition, and chelating ability. The PDP inhibited 53.09% of murine fibroblast (L929) and 57.58% of murine metastatic melanoma cells (B16–F10) at a concentration of 100 μg/mL and possesses an immunomodulatory effect by enhancing macrophage phagocytosis. These results offer a comprehensive understanding of the efficient extraction of PDP and their importance in considering their potential applications.