<p>Response surface methodology was employed to optimize the forward extraction of soy 11S globulin using sucrose-enhanced reverse micelles (RMs). Under optimal conditions (sucrose 0.012&#xa0;mol/L, W<sub>0</sub>, 18, pH 8.0, KCl 0.17&#xa0;mol/L, 45 °C, 30&#xa0;min, solid/liquid ratio 2.5&#xa0;g/L), the extraction yield reached 76.07%. Compared with alkaline extraction-isoelectric precipitation, the RMs-sucrose method significantly improved protein purity (96.81%), solubility (100%), emulsifying activity, foaming properties, water absorption (2.80&#xa0;g/g), and oil absorption (4.78&#xa0;g/g). While surface hydrophobicity and emulsifying stability were reduced, the RMs-sucrose systems system preserved protein structure and modified surface properties, enabling superior functional performance. This approach demonstrates potential for scalable extraction of high-functionality soy 11S globulin.</p>

错误:搜索内容不能为空,请输入英文关键词
错误:关键词超出字数限制,请精简
高级检索

Optimization of forward extraction and functional properties of soy 11S globulin by reverse micelles with chaotropic agents

  • Xue Sun,
  • Tao Fan,
  • Xiaoyan Zhao,
  • Hongkai Liu,
  • Meng Wang

摘要

Response surface methodology was employed to optimize the forward extraction of soy 11S globulin using sucrose-enhanced reverse micelles (RMs). Under optimal conditions (sucrose 0.012 mol/L, W0, 18, pH 8.0, KCl 0.17 mol/L, 45 °C, 30 min, solid/liquid ratio 2.5 g/L), the extraction yield reached 76.07%. Compared with alkaline extraction-isoelectric precipitation, the RMs-sucrose method significantly improved protein purity (96.81%), solubility (100%), emulsifying activity, foaming properties, water absorption (2.80 g/g), and oil absorption (4.78 g/g). While surface hydrophobicity and emulsifying stability were reduced, the RMs-sucrose systems system preserved protein structure and modified surface properties, enabling superior functional performance. This approach demonstrates potential for scalable extraction of high-functionality soy 11S globulin.