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<p>A comparative study of ultrasound-assisted extraction (UAE) was conducted, and for the first time, microwave-assisted extraction (MAE) was reported for the recovery of bioactive compounds from <i>Justicia spicigera.</i> Among the compounds identified in MAE were kaempferol and peonidin derivatives. In contrast, the extracts obtained via UAE contained cyanidin and kaempferol derivatives. These compounds were associated with the colorimetric changes observed as a function of pH. The results demonstrated that MAE achieved a higher recovery of phenolic compounds (48.93&#xa0;mg GAE/g of dry extract) and total flavonoids (23.46&#xa0;mg catechin/g of dry extract). Regarding antioxidant activity, MAE exhibited an IC<sub>50</sub> of 124.47&#xa0;μg of dry extract/mL in the DPPH assay, with no significant differences compared to ABTS and FRAP assays when compared to UAE. This study underscores the role of both extraction technologies in the recovery of bioactive compounds from <i>Justicia spicigera</i> and the potential development of active and intelligent packaging.</p>

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Comparative evaluation of bioactive compounds and antioxidant activity in Justicia spicigera extracts using ultrasound-assisted and microwave-assisted extraction methods

  • 期刊论文

  • 发表日期：

    2025年8月18日

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  • Alejandra Cristina Corona-Pérez,
  • Maria Fernanda Vargas-Torrico,
  • Miguel Angel Aguilar-Méndez,
  • Erich von Borries-Medrano

摘要

A comparative study of ultrasound-assisted extraction (UAE) was conducted, and for the first time, microwave-assisted extraction (MAE) was reported for the recovery of bioactive compounds from Justicia spicigera. Among the compounds identified in MAE were kaempferol and peonidin derivatives. In contrast, the extracts obtained via UAE contained cyanidin and kaempferol derivatives. These compounds were associated with the colorimetric changes observed as a function of pH. The results demonstrated that MAE achieved a higher recovery of phenolic compounds (48.93 mg GAE/g of dry extract) and total flavonoids (23.46 mg catechin/g of dry extract). Regarding antioxidant activity, MAE exhibited an IC50 of 124.47 μg of dry extract/mL in the DPPH assay, with no significant differences compared to ABTS and FRAP assays when compared to UAE. This study underscores the role of both extraction technologies in the recovery of bioactive compounds from Justicia spicigera and the potential development of active and intelligent packaging.