Scholarlink

<p>Illegal distribution and mislabeling of imported fishery products pose challenges to country-of-origin labeling regulations in Korea. To address this issue, a quantitative PCR (qPCR)-based method was developed to distinguish <i>Pagrus major</i> from Korea and Japan. Using genotyping-by-sequencing (GBS), two single nucleotide polymorphism (SNP) markers were identified, and allele-specific primers were designed. Gaussian mixture modeling established Ct thresholds, achieving accuracy of 81.67% and 77.78% for SNP001 and SNP008, respectively. The amplification efficiency and limit of detection (LOD) were assessed using tenfold serial dilutions (10–0.001&#xa0;ng/μL). Standard curves for AA and TT homotypes showed high linearity (R<sup>2</sup> &gt; 0.994) with amplification efficiencies of 103.65% and 97.63%, respectively. This qPCR-based method provides a reliable approach for origin verification of <i>P. major</i>, aiding regulatory enforcement and ensuring seafood authenticity.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A quantitative polymerase chain reaction approach for distinguishing the geographical origin of Pagrus major from Korea and Japan

  • 期刊论文

  • 发表日期：

    2025年4月10日

查看全文
  • Seung Hyun Yoo,
  • Jeong Gyu Kim,
  • Yong Jun Park,
  • Jong-Oh Kim,
  • Yong Bae Seo,
  • Gun-Do Kim

摘要

Illegal distribution and mislabeling of imported fishery products pose challenges to country-of-origin labeling regulations in Korea. To address this issue, a quantitative PCR (qPCR)-based method was developed to distinguish Pagrus major from Korea and Japan. Using genotyping-by-sequencing (GBS), two single nucleotide polymorphism (SNP) markers were identified, and allele-specific primers were designed. Gaussian mixture modeling established Ct thresholds, achieving accuracy of 81.67% and 77.78% for SNP001 and SNP008, respectively. The amplification efficiency and limit of detection (LOD) were assessed using tenfold serial dilutions (10–0.001 ng/μL). Standard curves for AA and TT homotypes showed high linearity (R2 > 0.994) with amplification efficiencies of 103.65% and 97.63%, respectively. This qPCR-based method provides a reliable approach for origin verification of P. major, aiding regulatory enforcement and ensuring seafood authenticity.