Scholarlink

<p>Mercury (Hg) poses a significant environmental and health threat, necessitating highly sensitive and rapid detection methods beyond costly conventional techniques. This study presents a novel electrochemical biosensor for Hg<sup>2+</sup> ions, leveraging the specific binding properties of the metalloregulatory protein, i.e., mercuric resistance operon regulatory protein (MerR), covalently immobilized on an Indium Tin Oxide (ITO) surface. We used electrochemically inert methoxy Polyethylene Glycol amino (MPA) as a blocking agent, avoiding the specificity issues associated with traditional Bovine Serum Albumin (BSA). Our MerR/ITO biosensor demonstrated exceptional specificity and achieved a limit of detection (LOD) of 0.77 nM. This performance represents a significant advancement for electrochemical mercury biosensors, particularly considering its robust and direct protein based detection mechanism. This approach offers a highly effective solution for precise mercury monitoring.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

MerR protein functionalized ITO electrode: an electrochemical biosensor for Mercury(II) detection

  • 期刊论文

  • 发表日期：

    2026年2月23日

查看全文
  • Ayushi Tanwar,
  • Debasish Nath,
  • Mandeep,
  • Saurabh Gupta,
  • Jaydeep Bhattacharya

摘要

Mercury (Hg) poses a significant environmental and health threat, necessitating highly sensitive and rapid detection methods beyond costly conventional techniques. This study presents a novel electrochemical biosensor for Hg2+ ions, leveraging the specific binding properties of the metalloregulatory protein, i.e., mercuric resistance operon regulatory protein (MerR), covalently immobilized on an Indium Tin Oxide (ITO) surface. We used electrochemically inert methoxy Polyethylene Glycol amino (MPA) as a blocking agent, avoiding the specificity issues associated with traditional Bovine Serum Albumin (BSA). Our MerR/ITO biosensor demonstrated exceptional specificity and achieved a limit of detection (LOD) of 0.77 nM. This performance represents a significant advancement for electrochemical mercury biosensors, particularly considering its robust and direct protein based detection mechanism. This approach offers a highly effective solution for precise mercury monitoring.