Purpose <p>Internet platforms, such as YouTube, play a significant role in disseminating health information. This study assessed the quality and reliability of YouTube videos related to ameloblastoma.</p> Methods <p>This cross-sectional study examined videos using the Global Quality Scale (GQS) to assess content quality and the DISCERN tool to determine reliability. The creators’ profiles and target audiences were also evaluated. Data were analyzed descriptively and analytically.</p> Results <p>A total of 65 videos were analyzed. Most videos (84.6%) were educational, while 15.4% were case reports. Specialists produced 40% of the videos, general dentists 21.5%, and students 6.2%. Regarding content quality, 23.2% of the videos received the highest GQS score, while 27.6% were rated as low quality (scores 1–2). According to DISCERN, 1.5% of videos were classified as maximum quality, 21.6% as high quality, and 17% as low quality. A significant difference in GQS scores was observed between oral and maxillofacial surgeons and general dentists (<i>p</i> = 0.025). A strong positive correlation was found between GQS and DISCERN scores (<i>r</i> = 0.79).</p> Conclusion <p>While most videos exhibited good production quality, their overall reliability was moderate, with notable deficiencies. Additional validation strategies are needed to enhance the accuracy and educational value of online health information.</p> Clinical trial <p>Not applicable.</p>