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<p>The bacterial phylum <i>Caldisericota</i> has a wide geographical and environmental distribution, but cultivated representatives of this phylum have so far been limited to a single strain. We have isolated a novel anaerobic, thermophilic, fermentative bacterium, strain AR60<sup>T</sup> from a thermal spring in Sakhalin Island, Russia. Cells of strain AR60<sup>T</sup> were long rods and tended to form aggregates. Growth was observed between 50 and 70&#xa0;°C (optimum at 60&#xa0;°C) and between pH 6.0 and 7.5 (optimum pH 6.5). The isolate was capable for fermentative growth on yeast extract, tryptone and peptone. Thiosulfate, S<sup>0</sup>, DMSO stimulated growth. The strain contained iso-C<sub>17:0</sub> as the major fatty acid. Genome size of strain AR60<sup>T</sup> was 1.58&#xa0;Mb; G + C content of the genomic DNA was 34.47%. Phylogenetic analysis revealed that strain AR60<sup>T</sup> belongs to the genus <i>Caldisericum</i> of the phylum <i>Caldisericota</i> and has 98.85% identity to <i>C. exile.</i> Based on physiological properties and phylogenetic analyses including an average nucleotide identity values and in silico DNA-DNA hybridization, strain AR60<sup>T</sup> is considered as a new species of the genus <i>Caldisericum.</i> We propose to name strain AR60<sup>T</sup> (= CGMCC 1.18100<sup>T</sup> = VKM B-3836<sup>T</sup>) <i>Caldisericum insulae</i> sp. nov. It is the second cultivated representative of the phylum <i>Caldisericota</i>.</p>

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Isolation and characterization of anaerobic thermophilic bacterium Caldisericum insulae sp.nov., the second cultivated representative of the phylum Caldisericota

  • 期刊论文

  • 发表日期：

    2026年2月3日

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  • Nataliya M. Ratnikova,
  • A. Y. Merkel,
  • A. A. Novikov,
  • A. I. Slobodkin

摘要

The bacterial phylum Caldisericota has a wide geographical and environmental distribution, but cultivated representatives of this phylum have so far been limited to a single strain. We have isolated a novel anaerobic, thermophilic, fermentative bacterium, strain AR60T from a thermal spring in Sakhalin Island, Russia. Cells of strain AR60T were long rods and tended to form aggregates. Growth was observed between 50 and 70 °C (optimum at 60 °C) and between pH 6.0 and 7.5 (optimum pH 6.5). The isolate was capable for fermentative growth on yeast extract, tryptone and peptone. Thiosulfate, S0, DMSO stimulated growth. The strain contained iso-C17:0 as the major fatty acid. Genome size of strain AR60T was 1.58 Mb; G + C content of the genomic DNA was 34.47%. Phylogenetic analysis revealed that strain AR60T belongs to the genus Caldisericum of the phylum Caldisericota and has 98.85% identity to C. exile. Based on physiological properties and phylogenetic analyses including an average nucleotide identity values and in silico DNA-DNA hybridization, strain AR60T is considered as a new species of the genus Caldisericum. We propose to name strain AR60T (= CGMCC 1.18100T = VKM B-3836T) Caldisericum insulae sp. nov. It is the second cultivated representative of the phylum Caldisericota.