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<p>Bacterial metal recovery is regarded as an environmentally friendly alternative to chemical hydrometallurgical waste metal recycling. However, conventional studies are generally conducted under neutral pH conditions, even though typical acidic metal leachate is strongly acidic. Previously, we isolated the bacterial strain <i>Priestia</i> sp. Mn7, which can grow under neutral pH and recover metals (Co, Cu, Li, Mn, and Ni) under pH 1.5. This study aimed to clarify this strain’s metal tolerance and recovery mechanisms under pH 1.5 conditions. Metal recovery test was conducted using pH 1.5 solution without the five metal species described above. Enhanced expression of genes related to the sugar ATP binding cassette transporter and increased sugar concentration in metal-abundant solution indicated that the strain expels sugar from the cell as a metal stress response under strongly acidic conditions. Dead cells did not recover metals except for Mn. Additionally, the recovered metals were distributed on the cell surface. These results indicated that the strain recovers tested metals via bioprecipitation related to sugar expulsion. To the best of our knowledge, this is the first study to elucidate the metal tolerance and recovery mechanisms of acid-tolerant bacteria under pH 1.5, further contributing to the understanding and utilization of acid-tolerant bacteria.</p>

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Metal tolerance and recovery mechanisms of Priestia sp. Mn7 under pH 1.5

  • 期刊论文

  • 发表日期：

    2025年10月28日

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  • Chikara Takano,
  • Kazunori Nakashima,
  • Hideki Aoyagi,
  • Takaaki Kojima,
  • Satoru Kawasaki

摘要

Bacterial metal recovery is regarded as an environmentally friendly alternative to chemical hydrometallurgical waste metal recycling. However, conventional studies are generally conducted under neutral pH conditions, even though typical acidic metal leachate is strongly acidic. Previously, we isolated the bacterial strain Priestia sp. Mn7, which can grow under neutral pH and recover metals (Co, Cu, Li, Mn, and Ni) under pH 1.5. This study aimed to clarify this strain’s metal tolerance and recovery mechanisms under pH 1.5 conditions. Metal recovery test was conducted using pH 1.5 solution without the five metal species described above. Enhanced expression of genes related to the sugar ATP binding cassette transporter and increased sugar concentration in metal-abundant solution indicated that the strain expels sugar from the cell as a metal stress response under strongly acidic conditions. Dead cells did not recover metals except for Mn. Additionally, the recovered metals were distributed on the cell surface. These results indicated that the strain recovers tested metals via bioprecipitation related to sugar expulsion. To the best of our knowledge, this is the first study to elucidate the metal tolerance and recovery mechanisms of acid-tolerant bacteria under pH 1.5, further contributing to the understanding and utilization of acid-tolerant bacteria.