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Objective <p>To examine the transdentinal response of odontoblast-like cells (MDPC-23), dental pulp stem cells (DPSC), and macrophages to flavonoid-based primers applied to caries-affected dentin.</p> Materials and methods <p>Primers were prepared from 20 mM of Naringin, Kaempferol, or Baicalein in 20% ethanol. Caries-affected dentin discs (<i>N</i> = 56) were produced using a microcosm biofilm model and mounted in artificial pulp chambers with MDPC-23 cells seeded on the pulp side. Occlusal surfaces (<i>n</i> = 8) were etched with 35% phosphoric acid, rinsed, and blot-dried, followed by application of ultrapure water (negative control, NC), 20% ethanol (solvent control, SC), flavonoid primers, or 29% hydrogen peroxide (positive control, PC). After 24&#xa0;h, cell viability and morphology (scanning electron microscopy, SEM) were evaluated. Extracts obtained by transdentinal diffusion were applied to DPSC and MDPC-23 cells for up to 7 days to assess cell viability and mineralization, and to macrophages (RAW 264.7) for reactive oxygen species (ROS) production. Data were analyzed using ANOVA and Sidak’s test (α = 5%).</p> Results <p>None of the flavonoid primers reduced MDPC-23 viability, while PC and phosphoric acid showed significant differences from NC. SEM images revealed altered cell morphology in acid-etched groups. After 7 days, primers slightly increased DPSC viability, maintained MDPC-23 viability, promoted mineralization, and reduced ROS.</p> Conclusions <p>Flavonoid-based primers applied to caries-affected dentin were non-cytotoxic, stimulated mineralization in pulp cells, and lowered oxidative stress in macrophages.</p> Clinical relevance <p>Flavonoid primers could support pulp health during dentin pretreatment, providing a conservative strategy for managing caries-affected dentin and reducing the risk of pulp irritation.</p>

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Transdentinal effects of flavonoid-based primers applied to caries-affected dentin on pulp cells

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月8日

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  • Beatriz Ometto Sahadi,
  • Igor Paulino Mendes Soares,
  • Chloe Gifford,
  • Caroline Anselmi,
  • Josimeri Hebling,
  • Marcelo Giannini,
  • Marco C. Bottino

摘要

Objective

To examine the transdentinal response of odontoblast-like cells (MDPC-23), dental pulp stem cells (DPSC), and macrophages to flavonoid-based primers applied to caries-affected dentin.

Materials and methods

Primers were prepared from 20 mM of Naringin, Kaempferol, or Baicalein in 20% ethanol. Caries-affected dentin discs (N = 56) were produced using a microcosm biofilm model and mounted in artificial pulp chambers with MDPC-23 cells seeded on the pulp side. Occlusal surfaces (n = 8) were etched with 35% phosphoric acid, rinsed, and blot-dried, followed by application of ultrapure water (negative control, NC), 20% ethanol (solvent control, SC), flavonoid primers, or 29% hydrogen peroxide (positive control, PC). After 24 h, cell viability and morphology (scanning electron microscopy, SEM) were evaluated. Extracts obtained by transdentinal diffusion were applied to DPSC and MDPC-23 cells for up to 7 days to assess cell viability and mineralization, and to macrophages (RAW 264.7) for reactive oxygen species (ROS) production. Data were analyzed using ANOVA and Sidak’s test (α = 5%).

Results

None of the flavonoid primers reduced MDPC-23 viability, while PC and phosphoric acid showed significant differences from NC. SEM images revealed altered cell morphology in acid-etched groups. After 7 days, primers slightly increased DPSC viability, maintained MDPC-23 viability, promoted mineralization, and reduced ROS.

Conclusions

Flavonoid-based primers applied to caries-affected dentin were non-cytotoxic, stimulated mineralization in pulp cells, and lowered oxidative stress in macrophages.

Clinical relevance

Flavonoid primers could support pulp health during dentin pretreatment, providing a conservative strategy for managing caries-affected dentin and reducing the risk of pulp irritation.