Scholarlink

Objective <p>To evaluate the influence of different surface roughness levels of titanium disks, induced by alumina blasting, on bacterial adhesion.</p> Materials and Methods <p>Twelve different surface roughnesses, ranging from 0.01&#xa0;μm to 6&#xa0;μm, were produced using a shot blasting technique with varying alumina particle sizes. Surface roughness was measured using confocal interferometry, wettability was assessed by contact angle measurements, and compressive residual stress was evaluated by X-ray diffraction. For each roughness level, 720 samples were used to culture Porphyromonas gingivalis (Gram-negative, anaerobic) and Streptococcus sanguinis (Gram-positive, anaerobic). The colonies formed per unit area, the ratio of dead bacteria to total bacteria, and the metabolic activity for each roughness ere determined.</p> Results <p>The polished surface (Sa = 0.01&#xa0;μm) showed the highest bacterial adhesion for both strains compared to the 0.13&#xa0;μm roughness, which exhibited a antibacterial activity, likely due to nanostructured peaks causing bacterial membrane disruption. For surface roughness values between 0.5 and 3&#xa0;μm, Gram-positive bacterial colonies increased approximately threefold. When the roughness exceeded 3.8&#xa0;μm, colony formation rose fivefold. In contrast, Gram-negative bacteria did not exhibit statistically significant changes in adhesion between 0.5 and 2&#xa0;μm. However, beginning at 2.6&#xa0;μm, a marked increase was observed, with colony numbers reaching nearly four times the control at 6&#xa0;μm. The ratio of dead bacteria and metabolic activity confirms bacterial colonization studies (CFU/mm<sup>2</sup>). </p> Conclusions <p>Surface roughness significantly influenced bacterial colonization on titanium implants. An antibacterial effect was observed at a roughness of 0.13&#xa0;μm. Bacterial adhesion increased moderately up to 2.1&#xa0;μm for Gram-negative and 3&#xa0;μm for Gram-positive strains, followed by a sharp rise at higher roughness values. An optimal surface roughness range of 1 to 2&#xa0;μm appears to promote favorable osteoblastic response while minimizing bacterial adhesion. </p> Clinical Relevance <p>These results enhance our understanding of how implant surface roughness influences bacterial adhesion. This knowledge could contribute to the development of clinical approaches designed to lower the risk of peri-implantitis</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Influence of alumina shot blasting induced roughness on bacterial adhesion to titanium

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月11日

查看全文
  • Marta Romero-Serrano,
  • Manuel María Romero-Ruiz,
  • José Vicente Ríos-Santos,
  • Blanca Ríos-Carrasco,
  • Javier Gil

摘要

Objective

To evaluate the influence of different surface roughness levels of titanium disks, induced by alumina blasting, on bacterial adhesion.

Materials and Methods

Twelve different surface roughnesses, ranging from 0.01 μm to 6 μm, were produced using a shot blasting technique with varying alumina particle sizes. Surface roughness was measured using confocal interferometry, wettability was assessed by contact angle measurements, and compressive residual stress was evaluated by X-ray diffraction. For each roughness level, 720 samples were used to culture Porphyromonas gingivalis (Gram-negative, anaerobic) and Streptococcus sanguinis (Gram-positive, anaerobic). The colonies formed per unit area, the ratio of dead bacteria to total bacteria, and the metabolic activity for each roughness ere determined.

Results

The polished surface (Sa = 0.01 μm) showed the highest bacterial adhesion for both strains compared to the 0.13 μm roughness, which exhibited a antibacterial activity, likely due to nanostructured peaks causing bacterial membrane disruption. For surface roughness values between 0.5 and 3 μm, Gram-positive bacterial colonies increased approximately threefold. When the roughness exceeded 3.8 μm, colony formation rose fivefold. In contrast, Gram-negative bacteria did not exhibit statistically significant changes in adhesion between 0.5 and 2 μm. However, beginning at 2.6 μm, a marked increase was observed, with colony numbers reaching nearly four times the control at 6 μm. The ratio of dead bacteria and metabolic activity confirms bacterial colonization studies (CFU/mm2).

Conclusions

Surface roughness significantly influenced bacterial colonization on titanium implants. An antibacterial effect was observed at a roughness of 0.13 μm. Bacterial adhesion increased moderately up to 2.1 μm for Gram-negative and 3 μm for Gram-positive strains, followed by a sharp rise at higher roughness values. An optimal surface roughness range of 1 to 2 μm appears to promote favorable osteoblastic response while minimizing bacterial adhesion.

Clinical Relevance

These results enhance our understanding of how implant surface roughness influences bacterial adhesion. This knowledge could contribute to the development of clinical approaches designed to lower the risk of peri-implantitis