Scholarlink

Objectives <p>This study aimed to evaluate doxycycline on the prevention of MRONJ.</p> Materials and methods <p>Seventy-two Wistar rats were sorted into 3 groups and subjected to tooth extractions. The first group received systemic saline solution (SAL), the second received zoledronic acid (ZA), and the third, besides the systemic ZA, the post-extraction socket received local application of doxycycline (DOXI) gel. Samples were collected and analyzed at 7, 14, and 28 postoperative days. <i>Results:</i> DOXI group presented higher percentage of vital bone area as compared with ZA at 7 (<i>p</i> = 0.0058), 14 (<i>p</i> &lt; 0.0001), and 28&#xa0;days (<i>p</i> &lt; 0.0001). TRAP immunolabeling was lower in ZA and DOXI at 7 and 14&#xa0;days, while OCN immunolabeling was similar between the ZA and DOXI, except at 7&#xa0;days. At 14 and 28&#xa0;days, DOXI group presented more collagen type 3 and 1 (<i>p</i> &lt; 0.0001) as compared with ZA. RUNX2, ALP, OCN, and RANKL gene expression was downregulated in ZA at 7&#xa0;days. Conversely, DOXI upregulated these markers and OPG over time (<i>p</i> &lt; 0.05).</p> Conclusion <p>Doxycycline is an effective local agent for preventing MRONJ, favoring alveolar bone healing. Clinical relevance.</p> <p>Although widely studied, MRONJ remains a significant challenge in dentistry. The search for preventive methods is important to assure safety for in patients under antiresorptive therapy that demand surgical dental treatment. Doxycycline is well-established in dentistry, and the proposal of using this medication as a gel makes it more appealing since it is applied locally and has negligible adverse effects.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The role of a local application of 10% doxycycline gel in the prevention of medication-related osteonecrosis of the jaw in rats

  • 期刊论文

  • 发表日期：

    2025年3月25日

查看全文
  • Henrique Hadad,
  • Laís Kawamata de Jesus,
  • Maísa Pereira-Silva,
  • Maria Eduarda de Freitas Santana Oliveira,
  • Naara Gabriela Monteiro,
  • Pier Paolo Poli,
  • Carlo Maiorana,
  • Roberta Okamoto,
  • Fernando Pozzi Semeghini Guastaldi,
  • Juliano Milanezi de Almeida,
  • Francisley Ávila Souza

摘要

Objectives

This study aimed to evaluate doxycycline on the prevention of MRONJ.

Materials and methods

Seventy-two Wistar rats were sorted into 3 groups and subjected to tooth extractions. The first group received systemic saline solution (SAL), the second received zoledronic acid (ZA), and the third, besides the systemic ZA, the post-extraction socket received local application of doxycycline (DOXI) gel. Samples were collected and analyzed at 7, 14, and 28 postoperative days. Results: DOXI group presented higher percentage of vital bone area as compared with ZA at 7 (p = 0.0058), 14 (p < 0.0001), and 28 days (p < 0.0001). TRAP immunolabeling was lower in ZA and DOXI at 7 and 14 days, while OCN immunolabeling was similar between the ZA and DOXI, except at 7 days. At 14 and 28 days, DOXI group presented more collagen type 3 and 1 (p < 0.0001) as compared with ZA. RUNX2, ALP, OCN, and RANKL gene expression was downregulated in ZA at 7 days. Conversely, DOXI upregulated these markers and OPG over time (p < 0.05).

Conclusion

Doxycycline is an effective local agent for preventing MRONJ, favoring alveolar bone healing. Clinical relevance.

Although widely studied, MRONJ remains a significant challenge in dentistry. The search for preventive methods is important to assure safety for in patients under antiresorptive therapy that demand surgical dental treatment. Doxycycline is well-established in dentistry, and the proposal of using this medication as a gel makes it more appealing since it is applied locally and has negligible adverse effects.