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Background <p>This study reported simultaneous estimation of kaempferol and vanillin in a binary mixture and formulation using Vierordt’s method, which is a cost-effective, precise, and accurate analytical method. Although vanillin and kaempferol have individually demonstrated strong therapeutic potential, no formulation has yet been developed. Moreover, a validated analytical method for their simultaneous estimation is currently lacking. This gap presents an opportunity to develop safer, more effective, and well-optimized therapeutic solutions through strategic advancements in both formulation design and analytical methodology.</p> Result <p>The procedure was validated as per the “International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH)” guidelines. The method demonstrated linearity for vanillin (R<sup>2</sup> = 0.9973) and kaempferol (R<sup>2</sup> = 0.9960), with low LOD/LOQ values confirming high sensitivity, enabling accurate simultaneous estimation of both compounds in a binary mixture using Vierordt’s method. Excellent accuracy with recovery values confirming reliability of the method for precise quantification. The method demonstrated excellent intraday and interday precision for both vanillin and kaempferol with %RSD values below 2%, confirming its reproducibility. Strong robustness with %RSD values below 0.65% under slight wavelength variations indicated consistent absorbance response and accuracy across tested concentrations. Excellent ruggedness with %RSD values below 2% was recorded for both the drugs, confirming reliability of the method under varied analytical conditions. Average percentage recovery of 100.012% for kaempferol and 98.884% for vanillin, confirming its applicability for simultaneous estimation in varied ratio combinations.</p> Conclusion <p>Based on the clear spectral separation, absence of interfering substances, and successful method validation, the selection of Vierordt’s method was considered scientifically justified and suitable for the intended purpose. Thus, the developed system can accurately identify vanillin and kaempferol in a binary mixture or formulation.</p>

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Validation of UV spectrophotometric method for simultaneous estimation of vanillin and kaempferol in a binary mixture and pharmaceutical dosage form

  • 期刊论文

  • 发表日期：

    2025年10月15日

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  • Vaishali Joshi,
  • Rajendra Awasthi

摘要

Background

This study reported simultaneous estimation of kaempferol and vanillin in a binary mixture and formulation using Vierordt’s method, which is a cost-effective, precise, and accurate analytical method. Although vanillin and kaempferol have individually demonstrated strong therapeutic potential, no formulation has yet been developed. Moreover, a validated analytical method for their simultaneous estimation is currently lacking. This gap presents an opportunity to develop safer, more effective, and well-optimized therapeutic solutions through strategic advancements in both formulation design and analytical methodology.

Result

The procedure was validated as per the “International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH)” guidelines. The method demonstrated linearity for vanillin (R2 = 0.9973) and kaempferol (R2 = 0.9960), with low LOD/LOQ values confirming high sensitivity, enabling accurate simultaneous estimation of both compounds in a binary mixture using Vierordt’s method. Excellent accuracy with recovery values confirming reliability of the method for precise quantification. The method demonstrated excellent intraday and interday precision for both vanillin and kaempferol with %RSD values below 2%, confirming its reproducibility. Strong robustness with %RSD values below 0.65% under slight wavelength variations indicated consistent absorbance response and accuracy across tested concentrations. Excellent ruggedness with %RSD values below 2% was recorded for both the drugs, confirming reliability of the method under varied analytical conditions. Average percentage recovery of 100.012% for kaempferol and 98.884% for vanillin, confirming its applicability for simultaneous estimation in varied ratio combinations.

Conclusion

Based on the clear spectral separation, absence of interfering substances, and successful method validation, the selection of Vierordt’s method was considered scientifically justified and suitable for the intended purpose. Thus, the developed system can accurately identify vanillin and kaempferol in a binary mixture or formulation.