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<p><i>Rosa laevigata</i> Michx., a traditional medicinal and edible plant in China, has been widely used for its health-promoting properties. This study aimed to develop a rapid and reliable analytical method for the simultaneous determination of eight bioactive triterpenoids in <i>R. laevigata</i> fruits and to explore their potential as functional food and pharmaceutical ingredients for the prevention of neurodegenerative diseases. Systematic optimization of HPLC conditions enabled quantitative analysis of triterpenoids within 40&#xa0;min, exhibiting excellent linearity, precision, stability, and reproducibility. Network pharmacology identified TNF and PPARG as the top two targets and revealed that 2<i>α</i>, 3<i>α</i>, 19<i>α</i>, 23-tetrahydroxyurs-12-en-28-oic acid exhibited multi-target affinity. Molecular docking and dynamics simulations further validated the stability of its binding with the target proteins. In vitro neuroprotection assays confirmed that 2<i>α</i>, 3<i>α</i>, 19<i>α</i>, 23-tetrahydroxyurs-12-en-28-oic acid significantly reversed H<sub>2</sub>O<sub>2</sub>-induced viability impairment in SH-SY5Y cells in a dose-dependent manner, consistent with predictions. This study not only provides a theoretical foundation for optimizing functional food and pharmaceutical formulations, but also highlights the potential of its natural components in neurodegenerative disease intervention through mechanistic exploration.</p>

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Quantitative analysis of triterpenoid characteristic components in Rosa laevigata Michx. using HPLC–UV and their potential as neuroprotective bioactive constituents

  • 期刊论文

  • 发表日期：

    2025年7月24日

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  • Yuhan Jin,
  • Yan Cheng,
  • Lingzhi Li,
  • Pinyi Gao,
  • Danqi Li,
  • Xuegui Liu

摘要

Rosa laevigata Michx., a traditional medicinal and edible plant in China, has been widely used for its health-promoting properties. This study aimed to develop a rapid and reliable analytical method for the simultaneous determination of eight bioactive triterpenoids in R. laevigata fruits and to explore their potential as functional food and pharmaceutical ingredients for the prevention of neurodegenerative diseases. Systematic optimization of HPLC conditions enabled quantitative analysis of triterpenoids within 40 min, exhibiting excellent linearity, precision, stability, and reproducibility. Network pharmacology identified TNF and PPARG as the top two targets and revealed that 2α, 3α, 19α, 23-tetrahydroxyurs-12-en-28-oic acid exhibited multi-target affinity. Molecular docking and dynamics simulations further validated the stability of its binding with the target proteins. In vitro neuroprotection assays confirmed that 2α, 3α, 19α, 23-tetrahydroxyurs-12-en-28-oic acid significantly reversed H2O2-induced viability impairment in SH-SY5Y cells in a dose-dependent manner, consistent with predictions. This study not only provides a theoretical foundation for optimizing functional food and pharmaceutical formulations, but also highlights the potential of its natural components in neurodegenerative disease intervention through mechanistic exploration.