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<p>In this work, a novel thin-layer chromatography (TLC) method for the simultaneous estimation of two biomarker compounds: ferulic acid (FA) and caffeic acid (CA) in the alcoholic extract of <i>Ipomoea carnea</i> Jacq. is established and validated. The developed method comprised a ternary mobile phase consisting of toluene–ethyl acetate–formic acid (5.5:4.5:0.5, <i>V/V</i>). Densitometric ultraviolet detection was carried out at 320&#xa0;nm, the absorption maxima of both markers. In compliance with the International Council for Harmonisation (ICH) criteria, the developed method was validated for precision, recovery, robustness, specificity, limit of detection, and limit of quantification. This mobile phase resulted in well resolved bands for CA and FA at <i>R</i><sub>F</sub> values of 0.44 and 0.51, respectively. The limit of detection (1.042 and 1.753&#xa0;ng), limit of quantification (3.160 and 5.310 ng), and recovery (86.05% and 90.85%) were satisfactory for both CA and FA, respectively. Future analysis and routine quality control of herbal materials and formulations containing <i>I. carnea</i> Jacq. may employ this new developed method. <i>I. carnea</i> parts (root, stem, leaf, flower, and whole plant) from five distinct geographic locations were also compared in this study using the developed method to investigate the influence of geographic regions on the accumulation of bioactive components.</p>

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Chromatographic estimation of caffeic acid and ferulic acid in different parts of Ipomoea carnea Jacq.: assessment of regional variance

  • 期刊论文

  • 发表日期：

    2026年3月20日

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  • Jyoti Dahiya,
  • Tuhin Baran Samoi,
  • Anupam Kumar Mangal,
  • Ankita Ghosh,
  • Ijaz Ahmed,
  • Nagayya Shiddamallayya,
  • Govindarajan Nartunai,
  • Gajji Babu,
  • Narayanam Srikanth,
  • Rabinarayan Acharya

摘要

In this work, a novel thin-layer chromatography (TLC) method for the simultaneous estimation of two biomarker compounds: ferulic acid (FA) and caffeic acid (CA) in the alcoholic extract of Ipomoea carnea Jacq. is established and validated. The developed method comprised a ternary mobile phase consisting of toluene–ethyl acetate–formic acid (5.5:4.5:0.5, V/V). Densitometric ultraviolet detection was carried out at 320 nm, the absorption maxima of both markers. In compliance with the International Council for Harmonisation (ICH) criteria, the developed method was validated for precision, recovery, robustness, specificity, limit of detection, and limit of quantification. This mobile phase resulted in well resolved bands for CA and FA at RF values of 0.44 and 0.51, respectively. The limit of detection (1.042 and 1.753 ng), limit of quantification (3.160 and 5.310 ng), and recovery (86.05% and 90.85%) were satisfactory for both CA and FA, respectively. Future analysis and routine quality control of herbal materials and formulations containing I. carnea Jacq. may employ this new developed method. I. carnea parts (root, stem, leaf, flower, and whole plant) from five distinct geographic locations were also compared in this study using the developed method to investigate the influence of geographic regions on the accumulation of bioactive components.