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<p>High-performance thin-layer chromatography combined with chemical or biological assay detections (multi-detection) is a powerful approach for identifying both known and unknown hazardous or beneficial compounds in complex samples. To spread the powerful technique, the latest do-it-yourself and open-source miniaturized 2LabsToGo-Eco provided a three-dimensional (3D)-printed piezoelectric Nebulizer for the homogeneous nebulization of organic as well as aqueous solutions and suspensions. However, the Nebulizer was restricted to 10&#xa0;cm × 10&#xa0;cm plates and operated by the 2LabsToGo-Eco software. Therefore, a stand-alone Nebulizer for plates up to 20&#xa0;cm × 10&#xa0;cm with integrated exhaust unit was developed for self-assembly as an affordable tool for the powerful planar effect-directed detection. In addition, it can also be connected to the 2LabsToGo-Eco. Its homogeneous and appropriate nebulization was demonstrated on various examples of fluorescence enhancer solutions, derivatization reagents, and bioassay detections.</p>

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Do-it-yourself, stand-alone, open-source nebulizer for derivatizations and bioassays in planar chromatography

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月2日

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  • Wolfgang Schwack,
  • Johannes Schmidt,
  • Thomas Nimmerfroh,
  • Gertrud E. Morlock

摘要

High-performance thin-layer chromatography combined with chemical or biological assay detections (multi-detection) is a powerful approach for identifying both known and unknown hazardous or beneficial compounds in complex samples. To spread the powerful technique, the latest do-it-yourself and open-source miniaturized 2LabsToGo-Eco provided a three-dimensional (3D)-printed piezoelectric Nebulizer for the homogeneous nebulization of organic as well as aqueous solutions and suspensions. However, the Nebulizer was restricted to 10 cm × 10 cm plates and operated by the 2LabsToGo-Eco software. Therefore, a stand-alone Nebulizer for plates up to 20 cm × 10 cm with integrated exhaust unit was developed for self-assembly as an affordable tool for the powerful planar effect-directed detection. In addition, it can also be connected to the 2LabsToGo-Eco. Its homogeneous and appropriate nebulization was demonstrated on various examples of fluorescence enhancer solutions, derivatization reagents, and bioassay detections.