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<p>Thin-layer chromatography (TLC) with capability for identifying and quantifying analytes, offers a cost-effective and quick approach to inform about <i>Artemisia annua</i> leaf quality in the field. This paper outlines the extraction of artemisinin and its quantification using TLC coupled with image analyzers and high-performance liquid chromatography (HPLC). For this purpose, the approach was validated using an artemisinin standard of concentrations, ranging from 2000&#xa0;to 100&#xa0;μg/mL, and the most reproducible of the settings assessed was applied to crude <i>A. annua</i> extracts. The results generated by JustTLC and TLC Analyzer were consistent with those from HPLC on applying corrective factors to account for systematic errors and/or differences in sensitivity of these analytical techniques, meaning these results compare well with HPLC.</p>

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JustTLC versus TLC Analyzer to inform about Artemisia annua leaves in the field—which won when benchmarked against high-performance liquid chromatography?

  • 期刊论文

  • 发表日期：

    2025年6月14日

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  • Adewale Olamoyesan,
  • Joshua Pilkington,
  • Rachel Gomes

摘要

Thin-layer chromatography (TLC) with capability for identifying and quantifying analytes, offers a cost-effective and quick approach to inform about Artemisia annua leaf quality in the field. This paper outlines the extraction of artemisinin and its quantification using TLC coupled with image analyzers and high-performance liquid chromatography (HPLC). For this purpose, the approach was validated using an artemisinin standard of concentrations, ranging from 2000 to 100 μg/mL, and the most reproducible of the settings assessed was applied to crude A. annua extracts. The results generated by JustTLC and TLC Analyzer were consistent with those from HPLC on applying corrective factors to account for systematic errors and/or differences in sensitivity of these analytical techniques, meaning these results compare well with HPLC.