Scholarlink

<p>The Siddha system is based on a combination of plant-based medicines and minerals along with the spiritual upliftment of the patient. One such formulation, Vilvam tablets, is indicated in controlling blood sugar levels in diabetics, renal calculi, cough, snake venom, and jaundice. This research uses the instrumental thin-layer chromatography (TLC) method to present the qualitative and quantitative analysis of mangiferin and ferulic acid in Vilvam tablets. The aluminum-backed precoated silica gel 60 F<sub>254</sub> plates were selected as the stationary phase, and the chromatographic separation was achieved with toluene‒ethyl acetate‒methanol‒formic acid (4.5:2.5:3:0.5, <i>V/V</i>). The wavelength for detection was 243&#xa0;nm. The symmetric compact peaks of mangiferin and ferulic acid were obtained at <i>R</i><sub>F</sub> of 0.30 ± 0.02 and 0.68 ± 0.02, respectively. All validation results indicated that the developed method was selective, precise, and accurate in the 200‒1100&#xa0;ng/band linearity range. Limit of detection and limit of quantification for mangiferin were 66.60 and 201.18&#xa0;ng, while for ferulic acid, they were 65.84 and 199.52&#xa0;ng. This method is robust and suitable for analyzing these two markers in pure and tablet dosage forms.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development and validation of an instrumental thin-layer chromatography method for the simultaneous estimation of mangiferin and ferulic acid in a Siddha formulation

  • 期刊论文

  • 发表日期：

    2025年3月13日

查看全文
  • Sneha Agrawal,
  • Hitesh Kalor,
  • Kajal Ghadge,
  • Gargi Patil,
  • Vishnavi Mohite,
  • Bhargavi Bunage,
  • Aruna Jadhav

摘要

The Siddha system is based on a combination of plant-based medicines and minerals along with the spiritual upliftment of the patient. One such formulation, Vilvam tablets, is indicated in controlling blood sugar levels in diabetics, renal calculi, cough, snake venom, and jaundice. This research uses the instrumental thin-layer chromatography (TLC) method to present the qualitative and quantitative analysis of mangiferin and ferulic acid in Vilvam tablets. The aluminum-backed precoated silica gel 60 F254 plates were selected as the stationary phase, and the chromatographic separation was achieved with toluene‒ethyl acetate‒methanol‒formic acid (4.5:2.5:3:0.5, V/V). The wavelength for detection was 243 nm. The symmetric compact peaks of mangiferin and ferulic acid were obtained at RF of 0.30 ± 0.02 and 0.68 ± 0.02, respectively. All validation results indicated that the developed method was selective, precise, and accurate in the 200‒1100 ng/band linearity range. Limit of detection and limit of quantification for mangiferin were 66.60 and 201.18 ng, while for ferulic acid, they were 65.84 and 199.52 ng. This method is robust and suitable for analyzing these two markers in pure and tablet dosage forms.