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<p>Indian mustard microgreens are recognized for their high nutritional value, being rich sources of vitamins, minerals, amino acids, and various bioactive compounds such as glucosinolates, phenolic compounds, and flavonoids, which have been linked to a variety of health advantages including antiinflammatory, antioxidant, and anticancer properties. Characterizing the metabolites present in mustard microgreens can help elucidate their potential health-promoting effects, thus aiding in dietary recommendations as well as guiding culinary practices for optimizing nutrient content and flavor. In the present study, we aimed to characterize the metabolites present in the methanolic extracts of mustard microgreens using high-field 800&#xa0;MHz NMR spectroscopy&#xa0;and liquid chromatography-mass spectrometry (LC-MS) based analysis. The composite use of 1D <sup>1</sup>H and 2D <sup>1</sup>H-<sup>13</sup>C HSQC, <sup>1</sup>H-<sup>1</sup>H TOCSY and J-resolved NMR spectral analysis employing commercial software CHENOMX led to the identification of 40 metabolites, including amino acids, organic acids, sugar molecules, γ-aminobutyric acid (GABA), and some phenolic compounds. LC–MS analysis provided complementary information, confirming the presence of key metabolites identified by NMR and revealing additional compounds not detected by NMR alone. The integrated analysis of NMR and LC–MS data enabled the comprehensive characterization of the metabolic profile of mustard microgreens, shedding light on their nutritional and medicinal properties.</p>

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Metabolic Characterization of Methanolic Extract of Indian Mustard Microgreens Using High-Resolution 800 MHz NMR Spectroscopy and LC–MS

  • 期刊论文

  • 发表日期：

    2026年3月24日

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  • Anamta Rizvi,
  • Sailendra Kumar,
  • Gurvinder Singh,
  • Dinesh Kumar,
  • Sangeeta Saxena

摘要

Indian mustard microgreens are recognized for their high nutritional value, being rich sources of vitamins, minerals, amino acids, and various bioactive compounds such as glucosinolates, phenolic compounds, and flavonoids, which have been linked to a variety of health advantages including antiinflammatory, antioxidant, and anticancer properties. Characterizing the metabolites present in mustard microgreens can help elucidate their potential health-promoting effects, thus aiding in dietary recommendations as well as guiding culinary practices for optimizing nutrient content and flavor. In the present study, we aimed to characterize the metabolites present in the methanolic extracts of mustard microgreens using high-field 800 MHz NMR spectroscopy and liquid chromatography-mass spectrometry (LC-MS) based analysis. The composite use of 1D 1H and 2D 1H-13C HSQC, 1H-1H TOCSY and J-resolved NMR spectral analysis employing commercial software CHENOMX led to the identification of 40 metabolites, including amino acids, organic acids, sugar molecules, γ-aminobutyric acid (GABA), and some phenolic compounds. LC–MS analysis provided complementary information, confirming the presence of key metabolites identified by NMR and revealing additional compounds not detected by NMR alone. The integrated analysis of NMR and LC–MS data enabled the comprehensive characterization of the metabolic profile of mustard microgreens, shedding light on their nutritional and medicinal properties.