<p>A critical and comprehensive analysis is presented on the current advancements in the detection and determination of coccidiostats (COCs) in food, feed, and environmental matrices, emphasizing emerging trends and analytical challenges. COCs, widely used in veterinary medicine, pose significant risks due to their potential toxicity, antimicrobial resistance concerns, and regulatory restrictions. Effective monitoring of COCs requires high-performance analytical techniques capable of addressing their structural diversity, low-concentration levels, and complex sample matrices. The review systematically evaluates state-of-the-art detection strategies, including electrochemical, optical, and separation-based approaches, with a focus on their analytical performance, applicability, and limitations. Advances in electrochemical techniques for COCs detection, including voltammetric, potentiometric, and amperometric methods, are assessed for their sensitivity and operational efficiency. Innovative optical approaches, such as spectrophotometric methods, fluorescence-based sensing, and surface-enhanced spectroscopy, are examined for their versatility and detection capabilities. Further, the utility of separation-based methods, including thin-layer chromatography, liquid and gas chromatography, and capillary electrophoresis, is discussed with respect to their applicability in complex, real-world matrices. Based on thorough evaluation of six decades of published research, this review synthesizes analytical advancements related to the detection and determination of 23 COCs, namely, amprolium, buquinolate, clopidol, cyromazin, decoquinate, diaveridine, diclazuril, dinitolmide, ethopabate, halofuginone, imidocarb, laidlomycin, lasalocid, maduramycin, monensin, narasin, nequinate, nicarbazin, robenidine, roxarsone, salinomycin, semduramicin, and toltrazuril.</p> Graphical Abstract <p></p>