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<p>Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne zoonotic pathogen. We evaluated nanopore adaptive sampling (NAS) for whole-genome assembly using two wildlife-derived SFTSV isolates (Vero cell-cultured) sequenced alongside tick-derived samples. Using the PromethION platform, complete L, M, and S segments were successfully assembled in a single run. NAS supported the reconstruction of previously characterized isolate, including an extended S segment, and enabled the characterization of a genotype B-2 isolate. These results suggest that NAS is an effective tool for genomic surveillance of SFTSV under complex sequencing conditions.</p>

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Whole-genome sequencing of severe fever with thrombocytopenia syndrome virus using nanopore adaptive sampling

  • 期刊论文

  • 发表日期：

    2026年5月25日

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  • Hye-ryung Byun,
  • Seong-Ryeong Ji,
  • Joon-Seok Chae

摘要

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne zoonotic pathogen. We evaluated nanopore adaptive sampling (NAS) for whole-genome assembly using two wildlife-derived SFTSV isolates (Vero cell-cultured) sequenced alongside tick-derived samples. Using the PromethION platform, complete L, M, and S segments were successfully assembled in a single run. NAS supported the reconstruction of previously characterized isolate, including an extended S segment, and enabled the characterization of a genotype B-2 isolate. These results suggest that NAS is an effective tool for genomic surveillance of SFTSV under complex sequencing conditions.