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<p>The giant freshwater prawn, <i>Macrobrachium rosenbergii</i>, has experienced significant economic losses due to the stunt growth in the past decade. In this study, random primer amplification was utilized in the slow-growth prawns to discover a novel <i>Macrobrachium rosenbergii</i> circular rep-encoding single-stranded (CRESS) DNA virus (MrCV) in Zhejiang, China. The MrCV genome is 2426 nt long, with two open reading frames (ORFs) encoding the replication protein (Rep) and capsid protein (Cp). The identity of the MrCV Cp (<i>&lt;</i> 56.23%) and Rep (&lt; 48.06%) sequence was compared to the reported CRESS DNA virus sequences. The existence of several highly conserved motifs of HUH endonuclease and superfamily 3 helicase domain in Rep protein were characterized and a GRS domain was found. Phylogenetic analysis of the Rep protein sequences and whole genome sequence revealed that MrCV is located in a branch consist of genomoviruses, circoviruses and unclassified viruses. A nested-PCR method was established to analyze the clinical slow-growth prawns, which were all MrCV-positive. Quantitative PCR results showed that MrCV viral load was significantly higher in the retarded growth prawns than that of sexual precocity, and presented a negative correlation to the prawn weights. The prevalence of MrCV in different life stages of <i>M</i>. <i>rosenbergii</i> was investigated and MrCV could be detected in zoea, postlarva and prawns. The present results reveal a high prevalence of MrCV in <i>M. rosenbergii</i> and suggest that this pathogen may associated with the retarded growth in <i>M. rosenbergii</i>.</p>

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Identification and full genome characterization of a novel CRESS DNA virus associated with giant freshwater prawn, Macrobrachium Rosenbergii

  • 期刊论文

  • 发表日期：

    2026年3月10日

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  • Qi Guo,
  • Yiniu Ji,
  • Weifeng Shen,
  • Bao Lou,
  • Sunjian Lyu,
  • Li Liu

摘要

The giant freshwater prawn, Macrobrachium rosenbergii, has experienced significant economic losses due to the stunt growth in the past decade. In this study, random primer amplification was utilized in the slow-growth prawns to discover a novel Macrobrachium rosenbergii circular rep-encoding single-stranded (CRESS) DNA virus (MrCV) in Zhejiang, China. The MrCV genome is 2426 nt long, with two open reading frames (ORFs) encoding the replication protein (Rep) and capsid protein (Cp). The identity of the MrCV Cp (< 56.23%) and Rep (< 48.06%) sequence was compared to the reported CRESS DNA virus sequences. The existence of several highly conserved motifs of HUH endonuclease and superfamily 3 helicase domain in Rep protein were characterized and a GRS domain was found. Phylogenetic analysis of the Rep protein sequences and whole genome sequence revealed that MrCV is located in a branch consist of genomoviruses, circoviruses and unclassified viruses. A nested-PCR method was established to analyze the clinical slow-growth prawns, which were all MrCV-positive. Quantitative PCR results showed that MrCV viral load was significantly higher in the retarded growth prawns than that of sexual precocity, and presented a negative correlation to the prawn weights. The prevalence of MrCV in different life stages of M. rosenbergii was investigated and MrCV could be detected in zoea, postlarva and prawns. The present results reveal a high prevalence of MrCV in M. rosenbergii and suggest that this pathogen may associated with the retarded growth in M. rosenbergii.