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<p>Alphabaculoviruses rel, in combination with natural putrefaction, on the auxiliary genes <i>v-cath</i> and <i>chiA</i> for host liquefaction, a process that is critical for virus release and horizontal transmission. Here, we describe DijuNPV-DF/23, a natural isolate infecting the passion fruit caterpillar <i>Dione juno</i> that fails to induce liquefaction. PCR amplification of the <i>v-cath/chiA</i> locus revealed a shorter amplicon compared to a previously reported virus, DijuNPV-Araguarina, suggesting gene disruption. Genome sequencing confirmed a 122,265-bp genome with 152 ORFs and a major structural alteration: <i>v-cath</i> was fragmented into two truncated ORFs due to a 76-nt deletion, while <i>chiA</i> remained intact. This provides a direct molecular explanation for the observed non-liquefying phenotype.</p>

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A natural isolate of Dione juno nucleopolyhedrovirus with a 76-bp deletion in the viral cathepsin-coding sequence fails to induce host liquefaction

  • 期刊论文

  • 发表日期：

    2026年1月7日

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  • Athos Campos Máximo-Branquinho,
  • Bergmann Morais Ribeiro,
  • Daniel M. P. Ardisson-Araújo

摘要

Alphabaculoviruses rel, in combination with natural putrefaction, on the auxiliary genes v-cath and chiA for host liquefaction, a process that is critical for virus release and horizontal transmission. Here, we describe DijuNPV-DF/23, a natural isolate infecting the passion fruit caterpillar Dione juno that fails to induce liquefaction. PCR amplification of the v-cath/chiA locus revealed a shorter amplicon compared to a previously reported virus, DijuNPV-Araguarina, suggesting gene disruption. Genome sequencing confirmed a 122,265-bp genome with 152 ORFs and a major structural alteration: v-cath was fragmented into two truncated ORFs due to a 76-nt deletion, while chiA remained intact. This provides a direct molecular explanation for the observed non-liquefying phenotype.