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<p>A novel phage specific for <i>Klebsiella pneumoniae</i>, named vB_KpnS_2146 − 302, was isolated from hospital wastewater. Through transmission electron microscopy observation and genomic analysis, this phage was found to belong to the genus <i>Webervirus</i> in the family <i>Drexlerviridae</i>. Biological studies showed that vB_KpnS_2146 − 302 has a narrow host range and exhibits specific lytic activity against <i>K. pneumoniae</i>. A one-step growth curve showed a latency period of 10 minutes and a burst size of 1125 PFU/cell. The phage remained stable within a pH range of 3–9 and a temperature range of 26–60°C. Whole-genome sequencing analysis showed that the double-stranded DNA genome of vB_KpnS_2146 − 302 is 50,299 bp in length and contains 76 open reading frames. Experiments in which cells were treated with proteinase K or periodate suggested that the carbohydrate structure of <i>K. pneumoniae</i> is involved in the adsorption of this phage. This study shows that phage vB_KpnS_2146 − 302 might be a new candidate for the development of phage therapy against <i>K. pneumoniae</i> infections.</p>

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Characterization and genome analysis of the Klebsiella webervirus vB_KpnS_2146−302

  • 期刊论文

  • 发表日期：

    2025年12月6日

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  • Huasheng Yu,
  • Bohai Du,
  • Hong Peng,
  • Xiao Wei,
  • Xiangna Zhao

摘要

A novel phage specific for Klebsiella pneumoniae, named vB_KpnS_2146 − 302, was isolated from hospital wastewater. Through transmission electron microscopy observation and genomic analysis, this phage was found to belong to the genus Webervirus in the family Drexlerviridae. Biological studies showed that vB_KpnS_2146 − 302 has a narrow host range and exhibits specific lytic activity against K. pneumoniae. A one-step growth curve showed a latency period of 10 minutes and a burst size of 1125 PFU/cell. The phage remained stable within a pH range of 3–9 and a temperature range of 26–60°C. Whole-genome sequencing analysis showed that the double-stranded DNA genome of vB_KpnS_2146 − 302 is 50,299 bp in length and contains 76 open reading frames. Experiments in which cells were treated with proteinase K or periodate suggested that the carbohydrate structure of K. pneumoniae is involved in the adsorption of this phage. This study shows that phage vB_KpnS_2146 − 302 might be a new candidate for the development of phage therapy against K. pneumoniae infections.