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<p>We tested whether rice root aphids (RRA; <i>Rhopalosiphum rufiabdominale</i>) and cannabis aphids (CAs; <i>Phorodon cannabis</i>) acquire hop latent viroid (HLVd) from infected cannabis plants (<i>Cannabis sativa</i>). One of eight groups of RRAs and four of eight groups of CAs feeding on infected plants tested positive for HLVd, as shown using a multiplex reverse transcription polymerase chain reaction assay. We found no evidence that HLVd-infected CAs transmitted HLVd to viroid-free cannabis plants, possibly because the amount of HLVd transmitted remained below the detection threshold. Further research is needed to determine whether CAs or RRAs are able to transmit HLVd to cannabis plants.</p>

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Acquisition of hop latent viroid from viroid-infected cannabis plants by rice root aphids and cannabis aphids

  • 期刊论文

  • 发表日期：

    2025年10月9日

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  • Mikhaela Ong,
  • Jack Munz,
  • Anita Feng,
  • Sauleha Yaqub,
  • Liam Buirs,
  • Gerhard Gries

摘要

We tested whether rice root aphids (RRA; Rhopalosiphum rufiabdominale) and cannabis aphids (CAs; Phorodon cannabis) acquire hop latent viroid (HLVd) from infected cannabis plants (Cannabis sativa). One of eight groups of RRAs and four of eight groups of CAs feeding on infected plants tested positive for HLVd, as shown using a multiplex reverse transcription polymerase chain reaction assay. We found no evidence that HLVd-infected CAs transmitted HLVd to viroid-free cannabis plants, possibly because the amount of HLVd transmitted remained below the detection threshold. Further research is needed to determine whether CAs or RRAs are able to transmit HLVd to cannabis plants.