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<p>Bovine astrovirus (BoAstV) belongs to the family <i>Astroviridae</i> and the genus <i>Mamastrovirus</i> (MAstV). Phylogenomic analysis based on partial sequences of the RNA-dependent RNA polymerase (RdRp) gene has allowed BoAstV isolates to be classified into the genotypes MAstV-13, 24, 28–30, and 33–35. However, information about the clinical significance and molecular characteristics of BoAstV from India is restricted to a single study from western Maharashtra, India. In this study, using RT-PCR detection of a portion of the RdRp gene, we investigated the presence of BoAstV in 134 rectal swabs samples from diarrheic and non-diarrheic calves less than three months of age from four states of North India (Rajasthan, Uttar Pradesh, Himachal Pradesh, and Haryana). Of these, 31 (23.1%) samples tested positive for BoAstV, with a higher rate of positivity in calves less than one month of age (30%, 21/70) than in calves between 1 and 3 months of age (15.6%, 10/64). Phylogenetic analysis based on partial RdRp gene sequences revealed the circulation of four different genotypes (MAstV 28, MAstV-33, MAstV-35, and MAstV-3) in Haryana. To the best of our knowledge, this is the first report of the presence of bovine astrovirus in cattle and buffalo calves in North India and of genotype MAstV-3 in calves.</p>

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Phylogenomic analysis of bovine astrovirus expands the known repertoire of mamastrovirus genotypes in northern India

  • 期刊论文

  • 发表日期：

    2025年8月16日

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  • Raman Mehtani,
  • Parveen Kumar,
  • Sanjeevna K. Minhas,
  • Ritu Panghal,
  • Anshul Lather,
  • Rajeev Kumar,
  • Puneet Singh,
  • Deepika Sheoran,
  • Akhil K. Gupta,
  • Rajesh Chhabra,
  • Naresh Jindal

摘要

Bovine astrovirus (BoAstV) belongs to the family Astroviridae and the genus Mamastrovirus (MAstV). Phylogenomic analysis based on partial sequences of the RNA-dependent RNA polymerase (RdRp) gene has allowed BoAstV isolates to be classified into the genotypes MAstV-13, 24, 28–30, and 33–35. However, information about the clinical significance and molecular characteristics of BoAstV from India is restricted to a single study from western Maharashtra, India. In this study, using RT-PCR detection of a portion of the RdRp gene, we investigated the presence of BoAstV in 134 rectal swabs samples from diarrheic and non-diarrheic calves less than three months of age from four states of North India (Rajasthan, Uttar Pradesh, Himachal Pradesh, and Haryana). Of these, 31 (23.1%) samples tested positive for BoAstV, with a higher rate of positivity in calves less than one month of age (30%, 21/70) than in calves between 1 and 3 months of age (15.6%, 10/64). Phylogenetic analysis based on partial RdRp gene sequences revealed the circulation of four different genotypes (MAstV 28, MAstV-33, MAstV-35, and MAstV-3) in Haryana. To the best of our knowledge, this is the first report of the presence of bovine astrovirus in cattle and buffalo calves in North India and of genotype MAstV-3 in calves.