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<p>A <i>Providencia</i> strain was isolated from farm effluents and used as a host to isolate a bacteriophage named A06. A double-layer agar plaque assay was used to determine the optimal multiplicity of infection (MOI), construct a one-step growth curve, evaluate the temperature and pH stability of the phage, and assess its <i>in vitro</i> antibacterial activity and lysis efficiency. Whole-genome sequencing, functional annotation, and comparative genomics analysis were used to analyze its biological features and determine its taxonomic position. Bacteriophage A06 has a circular DNA genome with 58 open reading frames (ORFs). The optimal MOI was determined to be 100, and a one-step growth curve showed the latent period to be 40 minutes and the lysis period to range from 40 to 90 minutes. Bacteriophage A06 exhibited a high degree of thermal stability and pH tolerance, and genomic analysis indicated that it represents a new species within the genus <i>Kakivirus</i> of the family <i>Autoscriptoviridae</i>.</p> Graphical abstract <p></p>

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Biological characterization and genomic profiling of a novel Providencia phage isolated from farm effluents

  • 期刊论文

  • 发表日期：

    2025年7月29日

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  • Mantong Zhu,
  • Chang Liu,
  • Guangfeng Liu,
  • Hongsai Zhang,
  • Xing Wang,
  • Ying Wang,
  • Yongqi Luo,
  • Jingzhe Jiang

摘要

A Providencia strain was isolated from farm effluents and used as a host to isolate a bacteriophage named A06. A double-layer agar plaque assay was used to determine the optimal multiplicity of infection (MOI), construct a one-step growth curve, evaluate the temperature and pH stability of the phage, and assess its in vitro antibacterial activity and lysis efficiency. Whole-genome sequencing, functional annotation, and comparative genomics analysis were used to analyze its biological features and determine its taxonomic position. Bacteriophage A06 has a circular DNA genome with 58 open reading frames (ORFs). The optimal MOI was determined to be 100, and a one-step growth curve showed the latent period to be 40 minutes and the lysis period to range from 40 to 90 minutes. Bacteriophage A06 exhibited a high degree of thermal stability and pH tolerance, and genomic analysis indicated that it represents a new species within the genus Kakivirus of the family Autoscriptoviridae.

Graphical abstract