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<p>Reverse genetics (RG) technology is useful for quickly updating influenza vaccine strains. A high-yield backbone (i.e., six segments other than hemagglutinin and neuraminidase) derived from A/Puerto Rico/8/1934 (PR8) has been developed to improve the growth of avian and human influenza viruses. However, for equine influenza virus (EIV), an EIV-derived backbone may have better growth properties due to more-natural segment combinations. We compared the growth properties in eggs between the PR8 backbone and an EIV backbone from A/equine/Ibaraki/1/2007, a vaccine strain in Japan. The results showed that the PR8 backbone was propagated more efficiently and is therefore more suitable for EIV vaccine production.</p>

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Growth properties of recombinant equine influenza viruses with different backbones generated by reverse genetics in embryonated chicken eggs

  • 期刊论文

  • 发表日期：

    2025年7月12日

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  • Manabu Nemoto,
  • Nanako Kawanishi,
  • Yoshinori Kambayashi,
  • Hiroshi Bannai,
  • Takashi Yamanaka,
  • Marie Garvey,
  • Ann Cullinane,
  • Seiya Yamayoshi,
  • Yoshihiro Kawaoka,
  • Koji Tsujimura

摘要

Reverse genetics (RG) technology is useful for quickly updating influenza vaccine strains. A high-yield backbone (i.e., six segments other than hemagglutinin and neuraminidase) derived from A/Puerto Rico/8/1934 (PR8) has been developed to improve the growth of avian and human influenza viruses. However, for equine influenza virus (EIV), an EIV-derived backbone may have better growth properties due to more-natural segment combinations. We compared the growth properties in eggs between the PR8 backbone and an EIV backbone from A/equine/Ibaraki/1/2007, a vaccine strain in Japan. The results showed that the PR8 backbone was propagated more efficiently and is therefore more suitable for EIV vaccine production.