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<p>A novel tri-segmented virus, tentatively named "Chenopodium trirhavirus 1" (CheTRV1), was identified in <i>Chenopodium album</i> in China using high-throughput sequencing combined with conventional Sanger sequencing after RT-PCR. The genome of CheTRV1 consists of three negative-sense single-stranded RNAs designated as RNA1, RNA2, and RNA3. RNAs 1–3 of CheTRV1 share 57.93–64.41%, 48.16–56.96%, and 38.12–42.22% nucleotide sequence identity with the corresponding segments of previously reported trirhaviruses. The 3′- and 5′-termini of the three segments are similar and exhibit partial inverse complementarity. The ORFs of CheTRV1 are separated by a conserved gene junction region with the consensus motif 3′-AAUUCUUUUGN(N)<sub>n</sub>UUC- 5′. RNA1 consists of 6749 nucleotides (nt) and encodes a single putative L protein. RNA2 is 4393 nt in length and contains four genes in the order 3′-N-P2-P3-P4-5′. The smaller RNA3 consists of 3897 nt and has three genes in the order 3′-P6-P7-P8-5′. Phylogenetic analysis and pairwise comparisons suggested that CheTRV1 is most closely related to Medicago trirhavirus 1, with 68.82% amino acid sequence identity in the L protein. This is the first report of the complete genome sequence of a tri-segmented rhabdovirus, and it expands our knowledge of rhabdovirus evolution.</p>

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Characterization of chenopodium trirhavirus 1, a novel tri-segmented virus of the family Rhabdoviridae

  • 期刊论文

  • 发表日期：

    2025年6月7日

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  • Xinyu Qin,
  • Zhihong Guo,
  • Zhangyao Nie,
  • Yizhou Yang,
  • Ruiqi Zhang,
  • Xianbing Wang,
  • Zongying Zhang,
  • Chenggui Han,
  • Ying Wang

摘要

A novel tri-segmented virus, tentatively named "Chenopodium trirhavirus 1" (CheTRV1), was identified in Chenopodium album in China using high-throughput sequencing combined with conventional Sanger sequencing after RT-PCR. The genome of CheTRV1 consists of three negative-sense single-stranded RNAs designated as RNA1, RNA2, and RNA3. RNAs 1–3 of CheTRV1 share 57.93–64.41%, 48.16–56.96%, and 38.12–42.22% nucleotide sequence identity with the corresponding segments of previously reported trirhaviruses. The 3′- and 5′-termini of the three segments are similar and exhibit partial inverse complementarity. The ORFs of CheTRV1 are separated by a conserved gene junction region with the consensus motif 3′-AAUUCUUUUGN(N)nUUC- 5′. RNA1 consists of 6749 nucleotides (nt) and encodes a single putative L protein. RNA2 is 4393 nt in length and contains four genes in the order 3′-N-P2-P3-P4-5′. The smaller RNA3 consists of 3897 nt and has three genes in the order 3′-P6-P7-P8-5′. Phylogenetic analysis and pairwise comparisons suggested that CheTRV1 is most closely related to Medicago trirhavirus 1, with 68.82% amino acid sequence identity in the L protein. This is the first report of the complete genome sequence of a tri-segmented rhabdovirus, and it expands our knowledge of rhabdovirus evolution.