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<p>Duck Tembusu virus (DTMUV) poses a significant threat to the waterfowl breeding industry in China. Here, we constructed a recombinant duck enteritis virus (DEV) expressing the entire E protein of DTMUV, named rDEV-ERT, using homologous recombination and the Cre-Lox system. The DTMUV E gene was integrated into the UL2 region of the DEV genome. At 14 days post-vaccination (dpv), 60 TCID<sub>50</sub> of rDEV-ERT provided complete protection against lethal DEV challenge. At 28 dpv, the positive rate of DTMUV antibodies induced by rDEV-ERT was 50%, and the protection rate against a virulent DTMUV strain was 62.5%. These findings support the development of a DEV-DTMUV recombinant live vector vaccine.</p>

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Construction and evaluation of a recombinant duck enteritis virus expressing the envelope protein of duck Tembusu virus in ducks

  • 期刊论文

  • 发表日期：

    2025年6月10日

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  • Lihua Wang,
  • Xiaoyue Yang,
  • Bing Zhang,
  • Siyuan Hao,
  • Yafen Song,
  • Dan Li,
  • Min Zhang,
  • Qianyi Zhang,
  • Yongda Zhao,
  • Chenghuai Yang

摘要

Duck Tembusu virus (DTMUV) poses a significant threat to the waterfowl breeding industry in China. Here, we constructed a recombinant duck enteritis virus (DEV) expressing the entire E protein of DTMUV, named rDEV-ERT, using homologous recombination and the Cre-Lox system. The DTMUV E gene was integrated into the UL2 region of the DEV genome. At 14 days post-vaccination (dpv), 60 TCID50 of rDEV-ERT provided complete protection against lethal DEV challenge. At 28 dpv, the positive rate of DTMUV antibodies induced by rDEV-ERT was 50%, and the protection rate against a virulent DTMUV strain was 62.5%. These findings support the development of a DEV-DTMUV recombinant live vector vaccine.