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<p>A novel mycovirus was identified in <i>Curvularia lunata</i> in China using high-throughput sequencing and RT-PCR, and the virus was named "Curvularia lunata RNA virus 1" (ClRV1). The genome of ClRV1 consists of 3,742 nucleotides and contains two open reading frames (ORFs). In a BLASTn search based on the complete genome sequence of ClRV1, this virus was found to have the most sequence similarity to Erysiphe necator associated umbra-like virus 4 (EnUlV4) (74% identity, 54% query coverage), and BLASTp searches showed that the ClRV1 HP and RdRp shared the highest amino acid sequence identity of 66.5% and 74.7%, respectively, with the corresponding proteins of EnUlV4. Phylogenetic analysis based on RdRp amino acid sequences indicated that ClRV1 grouped together with EnUlV4 and a number of other mycoviruses that have been variously named ‘ambiguiviruses’, ‘mycotombusviruses’, and ‘umbramycoviruses’, which appears to justify the creation of a new order within the phylum <i>Kitrinoviricota.</i> This is the first report of the complete genome sequence of the novel mycovirus ClRV1 infecting <i>Curvularia lunata</i>.</p>

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Molecular characterization of a novel mycovirus from Curvularia lunata in China

  • 期刊论文

  • 发表日期：

    2025年3月17日

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  • Bin Ke,
  • Xiaohan Mo,
  • Mengting Wu,
  • Lin Wang,
  • Ru Peng,
  • Yuwen Lu,
  • Jiejun Peng,
  • Shaofei Rao,
  • Guanwei Wu,
  • Jianping Chen,
  • Fei Yan,
  • Hongying Zheng

摘要

A novel mycovirus was identified in Curvularia lunata in China using high-throughput sequencing and RT-PCR, and the virus was named "Curvularia lunata RNA virus 1" (ClRV1). The genome of ClRV1 consists of 3,742 nucleotides and contains two open reading frames (ORFs). In a BLASTn search based on the complete genome sequence of ClRV1, this virus was found to have the most sequence similarity to Erysiphe necator associated umbra-like virus 4 (EnUlV4) (74% identity, 54% query coverage), and BLASTp searches showed that the ClRV1 HP and RdRp shared the highest amino acid sequence identity of 66.5% and 74.7%, respectively, with the corresponding proteins of EnUlV4. Phylogenetic analysis based on RdRp amino acid sequences indicated that ClRV1 grouped together with EnUlV4 and a number of other mycoviruses that have been variously named ‘ambiguiviruses’, ‘mycotombusviruses’, and ‘umbramycoviruses’, which appears to justify the creation of a new order within the phylum Kitrinoviricota. This is the first report of the complete genome sequence of the novel mycovirus ClRV1 infecting Curvularia lunata.