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<p>A positive-sense single-stranded RNA mycovirus was isolated from <i>Morchella sextelata</i> strain ZY-1 and designated as “Morchella sextelata endornavirus 1” (MsEV1). The complete genome of MsEV1 was found to be 16,589 nucleotides (nt) in length and to contain a single large open reading frame (ORF) encoding the viral proteins PRK00409, transmethylase, DEXDc, helicase, and RdRP in a polyprotein of 5,477 amino acids. Phylogenetic analysis based on RdRP nucleotide sequences revealed that this endornavirus is a member of the genus <i>Betaendornavirus</i> in the family <i>Endornaviridae</i>.</p>

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Characterization of a betaendornavirus isolated from the edible fungus Morchella sextelata

  • 期刊论文

  • 发表日期：

    2025年3月14日

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  • Ruiling Lyu,
  • Junlin Chen,
  • Qing Tang,
  • Du Hai,
  • Tun Wu,
  • Huagang Xiao,
  • Jiatao Xie,
  • Yang Xiao

摘要

A positive-sense single-stranded RNA mycovirus was isolated from Morchella sextelata strain ZY-1 and designated as “Morchella sextelata endornavirus 1” (MsEV1). The complete genome of MsEV1 was found to be 16,589 nucleotides (nt) in length and to contain a single large open reading frame (ORF) encoding the viral proteins PRK00409, transmethylase, DEXDc, helicase, and RdRP in a polyprotein of 5,477 amino acids. Phylogenetic analysis based on RdRP nucleotide sequences revealed that this endornavirus is a member of the genus Betaendornavirus in the family Endornaviridae.