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<p>In this study, we isolated a novel mycovirus from <i>Lasiodiplodia pseudotheobromae</i> strain YY-1, which we named “Lasiodiplodia pseudotheobromae mycovirus 1” (LpMyV1). The complete genome of LpMyV1 is 2877 bp in length and contains two non-overlapping open reading frames (ORF1 and ORF2). ORF1 encodes a putative protein of 316 amino acids with a molecular weight of 34.1 kDa that shares similarity with the coat proteins of several mycoviruses. ORF2 encodes a protein of 561 amino acids with a molecular weight of 63.2 kDa that contains a conserved RNA-dependent RNA polymerase (RdRp) domain. BLASTp results showed that the RdRp of LpMyV1 shares the highest similarity with those of non-segmented dsRNA viruses. A multiple sequence alignment and phylogenetic analysis indicated that LpMyV1 is a new member of the proposed genus “<i>Unirnavirus</i>”. This is the first report of a mycovirus from <i>L. pseudotheobromae</i>.</p>

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Molecular characterization of a novel non-segmented double-stranded RNA mycovirus isolated from the phytopathogenic fungus Lasiodiplodia pseudotheobromae

  • 期刊论文

  • 发表日期：

    2025年3月8日

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  • Zhengzhe Guan,
  • Mengjiao Wang,
  • Jiayi Ma,
  • Mengyuan Mu,
  • Xinyu Li,
  • Peimeng Sun,
  • Mengyuan Zhang,
  • Yuanyuan Zhang,
  • Luyang Song,
  • Caiyi Wen,
  • Ying Zhao

摘要

In this study, we isolated a novel mycovirus from Lasiodiplodia pseudotheobromae strain YY-1, which we named “Lasiodiplodia pseudotheobromae mycovirus 1” (LpMyV1). The complete genome of LpMyV1 is 2877 bp in length and contains two non-overlapping open reading frames (ORF1 and ORF2). ORF1 encodes a putative protein of 316 amino acids with a molecular weight of 34.1 kDa that shares similarity with the coat proteins of several mycoviruses. ORF2 encodes a protein of 561 amino acids with a molecular weight of 63.2 kDa that contains a conserved RNA-dependent RNA polymerase (RdRp) domain. BLASTp results showed that the RdRp of LpMyV1 shares the highest similarity with those of non-segmented dsRNA viruses. A multiple sequence alignment and phylogenetic analysis indicated that LpMyV1 is a new member of the proposed genus “Unirnavirus”. This is the first report of a mycovirus from L. pseudotheobromae.