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<p>The full genome sequence of a positive-sense (+) single-stranded (ss) RNA virus, which we have named "Neopestalotiopsis nebuloides deltaflexivirus 1" (NnDFV1), from <i>Neopestalotiopsis nebuloides</i> strain N-7 was sequenced and analyzed. The NnDFV1 genome is 7,719 nucleotides in length with a GC content of 49%, excluding the poly(A) tail, and contains a large open reading frame (ORF1) and three smaller ORFs (2–4). ORF1 encodes a replication-associated polyprotein (RP) consisting of three conserved domains: viral methyltransferase (Mtr), viral helicase (Hel), and RNA-dependent RNA polymerase (RdRp), whereas ORFs 2–4 encode three hypothetical proteins (18–20 kDa). Phylogenetic analysis showed that NnDFV1 formed a distinct clade together with Pestalotiopsis deltaflexivirus 1 (PDFV1), which is a new member of the genus <i>Deltaflexivirus</i> within the family <i>Deltaflexiviridae</i>. This is the first report of a novel deltaflexivirus found in the phytopathogenic fungus <i>Neopestalotiopsis nebuloides.</i></p>

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Molecular characterization of a novel deltaflexivirus from the plant-pathogenic fungus Neopestalotiopsis nebuloides strain N-7

  • 期刊论文

  • 发表日期：

    2025年2月8日

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  • Caiming Wu,
  • Siyu Zhou,
  • Changping Xie,
  • Daipeng Chen,
  • Li Zheng

摘要

The full genome sequence of a positive-sense (+) single-stranded (ss) RNA virus, which we have named "Neopestalotiopsis nebuloides deltaflexivirus 1" (NnDFV1), from Neopestalotiopsis nebuloides strain N-7 was sequenced and analyzed. The NnDFV1 genome is 7,719 nucleotides in length with a GC content of 49%, excluding the poly(A) tail, and contains a large open reading frame (ORF1) and three smaller ORFs (2–4). ORF1 encodes a replication-associated polyprotein (RP) consisting of three conserved domains: viral methyltransferase (Mtr), viral helicase (Hel), and RNA-dependent RNA polymerase (RdRp), whereas ORFs 2–4 encode three hypothetical proteins (18–20 kDa). Phylogenetic analysis showed that NnDFV1 formed a distinct clade together with Pestalotiopsis deltaflexivirus 1 (PDFV1), which is a new member of the genus Deltaflexivirus within the family Deltaflexiviridae. This is the first report of a novel deltaflexivirus found in the phytopathogenic fungus Neopestalotiopsis nebuloides.