Scholarlink

<p>An ultrasensitive electrochemiluminescence (ECL) biosensor was established by combining CRISPR/Cas12a technique and semiconductive bimetallic-organic framework (scMOF) [[Cu<sub>x</sub>Ni<sub>3−x</sub>(HITP)<sub>2</sub>] (HITP = 2,3,6,7,10,11-hexaiminotriphenylene)]] emitter and employed to detect <i>Salmonella</i> using the allosteric probe as the recognition component. Given that Cu<sub>x</sub>Ni<sub>3−x</sub>(HITP)<sub>2</sub> has demonstrated large specific surface area, both in-plane and out-of-plane charge transfer ability, narrowed band gap, and enhanced separation of holes and electrons, it can be simultaneously employed as the superior ECL emitter and bioplatform for anchoring single-strand DNA (ssDNA), thus improving the detection sensitivity toward <i>Salmonella</i>. The CRISPR/Cas12a-based system can specifically recognize the target sequence of <i>Salmonella</i> and activate the nuclease activity of Cas12a, and the activated Cas12a possesses trans-cleavage ability toward ssDNA. The Cu<sub>x</sub>Ni<sub>3−x</sub>(HITP)<sub>2</sub> emitter is then released, resulting in the decline of the ECL response. The developed Cu<sub>x</sub>Ni<sub>3−x</sub>(HITP)<sub>2</sub>-CRISPR/Cas12a-based ECL biosensor exhibits the ultralow detection limit of 0.25 CFU mL<sup>− 1</sup> in the linear range from 1.0 CFU mL<sup>− 1</sup> to 10<sup>6</sup> CFU mL<sup>− 1</sup>, significantly lower than those of reported ones. Furthermore, the developed biosensor exhibits outstanding overall biosensing properties with high selectivity, favorable reproducibility and stability, together with promising practical applicability for the determination of Salmonella in a variety of foodstuffs.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Ultrasensitive electrochemiluminescence determination of Salmonella based on CRISPR/Cas12a integrated with bimetallic semiconductive metal-organic frameworks

  • 期刊论文

  • 发表日期：

    2026年8月4日

查看全文
  • Liu Yang,
  • Xinxin Ji,
  • Zhaopeng Li,
  • Fenghe Duan,
  • Qiaojuan Jia,
  • Shuai Zhang,
  • Bin Hu,
  • Zhihong Zhang

摘要

An ultrasensitive electrochemiluminescence (ECL) biosensor was established by combining CRISPR/Cas12a technique and semiconductive bimetallic-organic framework (scMOF) [[CuxNi3−x(HITP)2] (HITP = 2,3,6,7,10,11-hexaiminotriphenylene)]] emitter and employed to detect Salmonella using the allosteric probe as the recognition component. Given that CuxNi3−x(HITP)2 has demonstrated large specific surface area, both in-plane and out-of-plane charge transfer ability, narrowed band gap, and enhanced separation of holes and electrons, it can be simultaneously employed as the superior ECL emitter and bioplatform for anchoring single-strand DNA (ssDNA), thus improving the detection sensitivity toward Salmonella. The CRISPR/Cas12a-based system can specifically recognize the target sequence of Salmonella and activate the nuclease activity of Cas12a, and the activated Cas12a possesses trans-cleavage ability toward ssDNA. The CuxNi3−x(HITP)2 emitter is then released, resulting in the decline of the ECL response. The developed CuxNi3−x(HITP)2-CRISPR/Cas12a-based ECL biosensor exhibits the ultralow detection limit of 0.25 CFU mL− 1 in the linear range from 1.0 CFU mL− 1 to 106 CFU mL− 1, significantly lower than those of reported ones. Furthermore, the developed biosensor exhibits outstanding overall biosensing properties with high selectivity, favorable reproducibility and stability, together with promising practical applicability for the determination of Salmonella in a variety of foodstuffs.

Graphical Abstract