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<p>A dual-mode aptasensor based on magnetic separation with a sequential signal conversion mechanism is developed for sensitive and specific detection of HepG2 cells. The system uses magnetic bead-labeled aptamers (MB-Apt, T1 strand), thiol-modified T2-functionalized silver nanoparticles (T2-AgNPs), and CdTe quantum dots (QDs). Without target cells, T2-AgNPs hybridize with MB-T1, leaving no AgNPs in the supernatant (no UV-vis absorption signal) while CdTe QDs produce strong fluorescence. Upon addition of HepG2 cells, aptamers bind to the cells, releasing AgNPs into the supernatant (UV-vis absorption signal). Subsequent acidolysis releases Ag⁺ ions, quenching QD fluorescence via cation exchange (FL signal). The dual signals offer self-validation and high anti-interference. Under optimal conditions, the fluorescence signal is linear from 0 to 2 × 10⁷ cells/mL (R²=0.985) and UV-vis absorption signal shows good linearity (R²=0.978). The sensor exhibits high specificity for HepG2 over other tumor cells. It successfully detects HepG2 cells spiked in human serum with satisfactory recoveries ranging from 96.2% to 102.5% for the fluorescence mode and 94.8% to 98.7% for the UV/colorimetric mode (RSDs &lt; 5.3%), demonstrating its potential for practical clinical application. </p> Graphical abstract <p></p>

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Dual-mode fluorescence and UV-vis aptasensor for hepatocellular carcinoma detection using magnetic separation

  • 期刊论文

  • 发表日期：

    2026年7月22日

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  • Yiwen Zheng,
  • Liquan Zhu,
  • Kang Yao,
  • Danhao Tao,
  • Chaoshen Wu,
  • Yibing Zhou,
  • Da Qian,
  • Jinbing Sun,
  • Chaoqi He

摘要

A dual-mode aptasensor based on magnetic separation with a sequential signal conversion mechanism is developed for sensitive and specific detection of HepG2 cells. The system uses magnetic bead-labeled aptamers (MB-Apt, T1 strand), thiol-modified T2-functionalized silver nanoparticles (T2-AgNPs), and CdTe quantum dots (QDs). Without target cells, T2-AgNPs hybridize with MB-T1, leaving no AgNPs in the supernatant (no UV-vis absorption signal) while CdTe QDs produce strong fluorescence. Upon addition of HepG2 cells, aptamers bind to the cells, releasing AgNPs into the supernatant (UV-vis absorption signal). Subsequent acidolysis releases Ag⁺ ions, quenching QD fluorescence via cation exchange (FL signal). The dual signals offer self-validation and high anti-interference. Under optimal conditions, the fluorescence signal is linear from 0 to 2 × 10⁷ cells/mL (R²=0.985) and UV-vis absorption signal shows good linearity (R²=0.978). The sensor exhibits high specificity for HepG2 over other tumor cells. It successfully detects HepG2 cells spiked in human serum with satisfactory recoveries ranging from 96.2% to 102.5% for the fluorescence mode and 94.8% to 98.7% for the UV/colorimetric mode (RSDs < 5.3%), demonstrating its potential for practical clinical application.

Graphical abstract