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<p>At-home monitoring of key blood indicators, including cholesterol and glucose, is vital for point-of-care chronic condition management. In this work, portable kits based on L-arginine modified Cu-CuFe<sub>2</sub>O<sub>4</sub> nanoparticles (Cu-CuFe<sub>2</sub>O<sub>4</sub>@L-Arg NPs) were fabricated for colorimetric determination of glucose and cholesterol in human serum. Cu-CuFe<sub>2</sub>O<sub>4</sub>@L-Arg NPs with superior peroxidase-like activities were synthesized <i>via</i> a straightforward hydrothermal approach. The accelerated electron transfer process of Cu<sup>0</sup> and its role as an electron donor contributed to excellent catalytic activity of Cu-CuFe<sub>2</sub>O<sub>4</sub>@L-Arg toward 3,3’,5,5’-tetramethylbenzidine (TMB). Portable kits include glucose oxidase or cholesterol oxidase, phosphate buffer solution (PBS) pH 7.0, Cu-CuFe<sub>2</sub>O<sub>4</sub>@L-Arg, PBS pH 4.0 and TMB. Among the enzyme-like reactions, Cu-CuFe<sub>2</sub>O<sub>4</sub>@L-Arg catalyzes the conversion of H<sub>2</sub>O<sub>2</sub> into hydroxyl radicals (•OH), which subsequently oxidize the TMB substrate to yield a blue-colored product. The signal intensities of the blue products were proportional to H<sub>2</sub>O<sub>2</sub>, which was produced in the cholesterol or glucose systems. This method achieves linear detection of free cholesterol from 5 µM to 1.2 mM, exhibiting a low LOD of 1 µM, and was validated by obtaining recoveries of 97-103.5% for total cholesterol in human serum analyses. The method enabled the linear quantification of glucose from 50 to 1500 µM with a limit of detection (LOD) of 5 µM, and recoveries in human serum ranged from 96% to 102.8%. The portable kits, characterized by low cost, visual readout, high specificity, and long-term stability, demonstrate considerable potential for self-monitoring devices for key clinical biomarkers.</p> Graphical Abstract <p></p>

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Portable kits based on L-arginine modified Cu-CuFe2O4 with superior peroxidase-like activity for colorimetric detection of cholesterol and glucose in human serum

  • 期刊论文

  • 发表日期：

    2026年7月1日

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  • Zexing Xie,
  • Xinkai Dou,
  • Haitong Wang,
  • Yu Liu,
  • Peiwen Lv,
  • Tao Xin,
  • Nianlu Li

摘要

At-home monitoring of key blood indicators, including cholesterol and glucose, is vital for point-of-care chronic condition management. In this work, portable kits based on L-arginine modified Cu-CuFe2O4 nanoparticles (Cu-CuFe2O4@L-Arg NPs) were fabricated for colorimetric determination of glucose and cholesterol in human serum. Cu-CuFe2O4@L-Arg NPs with superior peroxidase-like activities were synthesized via a straightforward hydrothermal approach. The accelerated electron transfer process of Cu0 and its role as an electron donor contributed to excellent catalytic activity of Cu-CuFe2O4@L-Arg toward 3,3’,5,5’-tetramethylbenzidine (TMB). Portable kits include glucose oxidase or cholesterol oxidase, phosphate buffer solution (PBS) pH 7.0, Cu-CuFe2O4@L-Arg, PBS pH 4.0 and TMB. Among the enzyme-like reactions, Cu-CuFe2O4@L-Arg catalyzes the conversion of H2O2 into hydroxyl radicals (•OH), which subsequently oxidize the TMB substrate to yield a blue-colored product. The signal intensities of the blue products were proportional to H2O2, which was produced in the cholesterol or glucose systems. This method achieves linear detection of free cholesterol from 5 µM to 1.2 mM, exhibiting a low LOD of 1 µM, and was validated by obtaining recoveries of 97-103.5% for total cholesterol in human serum analyses. The method enabled the linear quantification of glucose from 50 to 1500 µM with a limit of detection (LOD) of 5 µM, and recoveries in human serum ranged from 96% to 102.8%. The portable kits, characterized by low cost, visual readout, high specificity, and long-term stability, demonstrate considerable potential for self-monitoring devices for key clinical biomarkers.

Graphical Abstract