Scholarlink

<p>Conventional enantioseparation approaches of aromatic amino acids (AAAs) commonly suffer from inherent limitations, including insufficient detection sensitivity, poor enantioselectivity, and inefficient analyte preconcentration, which severely restrict their application in trace-level determinations. We rationally designed a metal–organic framework (MOF)-modified capillary electrochromatography (CEC) system by integrating MIL-140&#xa0;C as an efficient enrichment material and HQA-MOF as a high-performance chiral stationary phase. This integrated platform achieved up to 120-fold analyte enrichment and a low limit of detection (LOD) of 12.5 ng/mL, exhibiting significantly improved detection sensitivity, chiral resolution, and method reproducibility compared to traditional strategies. Method validation in spiked bovine serum albumin (BSA) samples demonstrated satisfactory recoveries ranging from 92.35% to 108.04%. The established MOF-CEC strategy provides a highly sensitive, selective, and scalable analytical tool for the precise chiral determination of trace Dansylated Aromatic Amino Acids (DNS-AAAs) in simulated biological samples.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Enhanced online preconcentration and enantioseparation of MOF-coated capillary electrochromatography for chiral derivatized aromatic amino acids

  • 期刊论文

  • 发表日期：

    2026年5月23日

查看全文
  • Chengxi Liu,
  • Dan Zhong,
  • Li Qi,
  • Xiangtai Zheng,
  • Yang Long,
  • Shihan Xiao,
  • Danni Zhang,
  • Zizhen Liu,
  • Jing Wang,
  • Wenjie Liu,
  • Wei Li,
  • Xiaodong Sun

摘要

Conventional enantioseparation approaches of aromatic amino acids (AAAs) commonly suffer from inherent limitations, including insufficient detection sensitivity, poor enantioselectivity, and inefficient analyte preconcentration, which severely restrict their application in trace-level determinations. We rationally designed a metal–organic framework (MOF)-modified capillary electrochromatography (CEC) system by integrating MIL-140 C as an efficient enrichment material and HQA-MOF as a high-performance chiral stationary phase. This integrated platform achieved up to 120-fold analyte enrichment and a low limit of detection (LOD) of 12.5 ng/mL, exhibiting significantly improved detection sensitivity, chiral resolution, and method reproducibility compared to traditional strategies. Method validation in spiked bovine serum albumin (BSA) samples demonstrated satisfactory recoveries ranging from 92.35% to 108.04%. The established MOF-CEC strategy provides a highly sensitive, selective, and scalable analytical tool for the precise chiral determination of trace Dansylated Aromatic Amino Acids (DNS-AAAs) in simulated biological samples.

Graphical abstract