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<p> A novel fireproof material@UIO-66-gold nanoparticles (AS@UIO-66-AuNPs) fiber network using low-cost fireproof material (AS) via an in-situ synthesis strategy has been developed. This functionalized network anchors specific aptamers (Apt), creating a highly efficient aptamer affinity column for the sensitive detection of aflatoxin B1 (AFB1) in food samples. Notably, this work focuses on the construction of an aptamer-based affinity pretreatment column rather than a signal-output sensing platform, aiming to improve target enrichment and matrix cleanup prior to instrumental analysis. Under optimized conditions, the method demonstrated excellent analytical performance, with a linearity range of 1–100&#xa0;µg/kg and a detection limit of 0.157&#xa0;µg/kg. Validation with spiked recovery experiments on corn, soybean meal, and rapeseed meal showed satisfactory recoveries and strong agreement with a commercial detection kit. The method also exhibited excellent reproducibility and reusability. Overall, the AS@UIO-66-AuNP/Apt system represents a cost-effective, stable, and selective strategy for the pretreatment and enrichment of AFB1, with potential applicability to other targets through aptamer replacement, thereby supporting the development of multifunctional aptamer affinity columns.</p> Graphical abstract <p></p>

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Aptamer-modified metal-organic framework fiber network as a selective and recyclable affinity pretreatment column for aflatoxin B1 enrichment in feed

  • 期刊论文

  • 发表日期：

    2026年4月13日

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  • Xia Lu,
  • Long Wang,
  • Qinyi Gao,
  • Xianglin Zhang,
  • Yuxin Wang,
  • Fang Wang,
  • Xujin Yang,
  • Wupeng Ge

摘要

A novel fireproof material@UIO-66-gold nanoparticles (AS@UIO-66-AuNPs) fiber network using low-cost fireproof material (AS) via an in-situ synthesis strategy has been developed. This functionalized network anchors specific aptamers (Apt), creating a highly efficient aptamer affinity column for the sensitive detection of aflatoxin B1 (AFB1) in food samples. Notably, this work focuses on the construction of an aptamer-based affinity pretreatment column rather than a signal-output sensing platform, aiming to improve target enrichment and matrix cleanup prior to instrumental analysis. Under optimized conditions, the method demonstrated excellent analytical performance, with a linearity range of 1–100 µg/kg and a detection limit of 0.157 µg/kg. Validation with spiked recovery experiments on corn, soybean meal, and rapeseed meal showed satisfactory recoveries and strong agreement with a commercial detection kit. The method also exhibited excellent reproducibility and reusability. Overall, the AS@UIO-66-AuNP/Apt system represents a cost-effective, stable, and selective strategy for the pretreatment and enrichment of AFB1, with potential applicability to other targets through aptamer replacement, thereby supporting the development of multifunctional aptamer affinity columns.

Graphical abstract