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<p>The establishment of a fast and precise method for glyphosate detection holds critical importance in ensuring food safety. Herein, a multimodal sensing platform that integrated UV-vis, fluorescence and smartphone-assisted portable hydrogel kit was applied to detect glyphosate relying on the light-responsive oxidase-mimicking activity and intrinsic fluorescence of carbon dots (C-dots). C-dots enable the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), resulting in blue-colored oxidized TMB (oxTMB) along with enhanced absorbance at 652&#xa0;nm. Simultaneously, the inner filter effect between oxTMB and C-dots induces the fluorescence quenching of C-dots. However, the introduction of copper ions can capture photogenerated electrons, thereby inhibiting the oxidase-mimicking catalytic activity of C-dots, leading to a decrease in absorbance and the restoration of fluorescence. Once glyphosate is present in the system, it can coordinate with copper ions to restore the catalytic activity of C-dots, thereby causing an increase in absorbance and a simultaneous decrease in fluorescence. Consequently, quantification detection of glyphosate can be realized via UV-vis and fluorescence modes. More importantly, in light of the changes in solution color, a smartphone-assisted portable hydrogel kit was also developed for glyphosate detection. Therefore, a UV-vis-fluorescence-smartphone-assisted hydrogel “three-in-one” platform was established for glyphosate determination with corresponding limits of detection of&#xa0;0.31, 0.12 and 5.07&#xa0;µg/mL, respectively. This platform can achieve glyphosate detection within just one minute. Notably, this work represents the first application of light-responsive C-dots oxidase mimetics for glyphosate detection. By utilizing visible light as a clean energy source to drive and regulate the catalytic reaction, the proposed strategy eliminates the dependence on hazardous H₂O₂, offering a green, accurate, and rapid sensing platform with broad application.</p> Graphical Abstract <p></p>

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A UV-vis-fluorescence-smartphone-assisted hydrogel “three-in-one” platform based on light-responsive carbon dots nanozyme for multimodal glyphosate detection

  • 期刊论文

  • 发表日期：

    2026年4月29日

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  • Wanqi Yang,
  • Haodong Yang,
  • Yaxin Ma,
  • Ao Hou,
  • Xiangyu Yang,
  • Pengjuan Ni,
  • Yizhong Lu

摘要

The establishment of a fast and precise method for glyphosate detection holds critical importance in ensuring food safety. Herein, a multimodal sensing platform that integrated UV-vis, fluorescence and smartphone-assisted portable hydrogel kit was applied to detect glyphosate relying on the light-responsive oxidase-mimicking activity and intrinsic fluorescence of carbon dots (C-dots). C-dots enable the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), resulting in blue-colored oxidized TMB (oxTMB) along with enhanced absorbance at 652 nm. Simultaneously, the inner filter effect between oxTMB and C-dots induces the fluorescence quenching of C-dots. However, the introduction of copper ions can capture photogenerated electrons, thereby inhibiting the oxidase-mimicking catalytic activity of C-dots, leading to a decrease in absorbance and the restoration of fluorescence. Once glyphosate is present in the system, it can coordinate with copper ions to restore the catalytic activity of C-dots, thereby causing an increase in absorbance and a simultaneous decrease in fluorescence. Consequently, quantification detection of glyphosate can be realized via UV-vis and fluorescence modes. More importantly, in light of the changes in solution color, a smartphone-assisted portable hydrogel kit was also developed for glyphosate detection. Therefore, a UV-vis-fluorescence-smartphone-assisted hydrogel “three-in-one” platform was established for glyphosate determination with corresponding limits of detection of 0.31, 0.12 and 5.07 µg/mL, respectively. This platform can achieve glyphosate detection within just one minute. Notably, this work represents the first application of light-responsive C-dots oxidase mimetics for glyphosate detection. By utilizing visible light as a clean energy source to drive and regulate the catalytic reaction, the proposed strategy eliminates the dependence on hazardous H₂O₂, offering a green, accurate, and rapid sensing platform with broad application.

Graphical Abstract