Scholarlink

<p>Antimicrobial peptides (AMPs) are critical effectors of innate immunity and promising alternatives to conventional antibiotics. However, their low abundance and interference from high-molecular-weight proteins in biological matrices hinder their selective enrichment and detection. Here, we report the design and synthesis of dual-pore covalent organic framework (COF)-shelled magnetic particles (M-Si@COF12) for efficient AMP and microprotein enrichment. The particles consist of magnetic core coated with silica (M-Si), followed by room-temperature, silanol-initiated growth of hair-like COF structures. This unique design provides precise control over hydrophobicity and dual pore sizes (2.7 and 3.2&#xa0;nm), facilitating both size-selective and affinity-driven capture of low-molecular-weight bioactive peptides. M-Si@COF12 exhibits a high surface area (1014.95&#xa0;m²/g), strong adsorption capacity (39.36&#xa0;mg/g), and excellent selectivity toward AMPs. The material was validated using human serum and K-562 cell lysate, demonstrating effective enrichment of AMPs and AMP-like hydrophobic peptides (microprotein mimics). The particles are also reusable without significant loss of performance. This work presents a facile, scalable, and reusable material platform for targeted enrichment of bioactive microproteins, establishing a potential foundation for future applications in clinical proteomics and biomarker discovery.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Dual-pore size covalent organic frameworks shelled magnetic particles for anti-microbial peptide and microprotein enrichment with simultaneous exclusion of large proteins

  • 期刊论文

  • 发表日期：

    2026年3月24日

查看全文
  • Irfan Azhar,
  • Aaiza Gul,
  • Arshad Ali,
  • Muhammad Imran,
  • Iram Yasmin

摘要

Antimicrobial peptides (AMPs) are critical effectors of innate immunity and promising alternatives to conventional antibiotics. However, their low abundance and interference from high-molecular-weight proteins in biological matrices hinder their selective enrichment and detection. Here, we report the design and synthesis of dual-pore covalent organic framework (COF)-shelled magnetic particles (M-Si@COF12) for efficient AMP and microprotein enrichment. The particles consist of magnetic core coated with silica (M-Si), followed by room-temperature, silanol-initiated growth of hair-like COF structures. This unique design provides precise control over hydrophobicity and dual pore sizes (2.7 and 3.2 nm), facilitating both size-selective and affinity-driven capture of low-molecular-weight bioactive peptides. M-Si@COF12 exhibits a high surface area (1014.95 m²/g), strong adsorption capacity (39.36 mg/g), and excellent selectivity toward AMPs. The material was validated using human serum and K-562 cell lysate, demonstrating effective enrichment of AMPs and AMP-like hydrophobic peptides (microprotein mimics). The particles are also reusable without significant loss of performance. This work presents a facile, scalable, and reusable material platform for targeted enrichment of bioactive microproteins, establishing a potential foundation for future applications in clinical proteomics and biomarker discovery.

Graphical Abstract