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<p>An innovative and highly sensitive quenching-type electrochemiluminescence (ECL) immunosensor for the detection of hemagglutinin, a key surface protein of the influenza A (H1N1) virus, is presented. For the first time, a composite of cobalt-iron bimetallic organic framework loaded with gold nanoparticles (Au NPs/Co-Fe-MOF), exhibiting high peroxidase-like activity and capable of significantly enhancing the initial ECL signal in a H<sub>2</sub>O<sub>2</sub> system, is combined with a composite of zirconium-based metal-organic framework loaded with copper oxide nanoparticles (CuO@UiO-66-NH<sub>2</sub>). The high overlap between the ultraviolet absorption spectrum of CuO@UiO-66-NH<sub>2</sub> and the emission spectrum of luminol allows for a substantial reduction in the initial signal via ECL resonance energy transfer. Under optimized conditions, the sensor exhibited a linear range from 0.001 to 80 ng mL⁻<sup>1</sup>, with a detection limit of 0.00047 ng mL⁻<sup>1</sup>. Moreover, no significant cross-reactivity with related viruses was observed, demonstrating excellent specificity and good stability. This easy-to-operate method is expected to offer a new approach with strong application potential for detecting H1N1 influenza virus.</p> Graphical abstract <p></p>

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A quenched electrochemiluminescence immunosensor based on luminol-Au NPs/Co-Fe-MOF and CuO@UiO-66-NH2 for influenza A hemagglutinin detection

  • 期刊论文

  • 发表日期：

    2026年3月18日

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  • Xinyi Yu,
  • Yuzhu Dai,
  • Jun Cheng,
  • Zhu Wang,
  • Haoyuan Wu,
  • Tingting Ye,
  • Yumei Ge,
  • Weizhong Wang,
  • Zhen Guo

摘要

An innovative and highly sensitive quenching-type electrochemiluminescence (ECL) immunosensor for the detection of hemagglutinin, a key surface protein of the influenza A (H1N1) virus, is presented. For the first time, a composite of cobalt-iron bimetallic organic framework loaded with gold nanoparticles (Au NPs/Co-Fe-MOF), exhibiting high peroxidase-like activity and capable of significantly enhancing the initial ECL signal in a H2O2 system, is combined with a composite of zirconium-based metal-organic framework loaded with copper oxide nanoparticles (CuO@UiO-66-NH2). The high overlap between the ultraviolet absorption spectrum of CuO@UiO-66-NH2 and the emission spectrum of luminol allows for a substantial reduction in the initial signal via ECL resonance energy transfer. Under optimized conditions, the sensor exhibited a linear range from 0.001 to 80 ng mL⁻1, with a detection limit of 0.00047 ng mL⁻1. Moreover, no significant cross-reactivity with related viruses was observed, demonstrating excellent specificity and good stability. This easy-to-operate method is expected to offer a new approach with strong application potential for detecting H1N1 influenza virus.

Graphical abstract