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<p>A novel dual-channel core-shell-shell up-conversion nanoparticle-based fluorescence immunochromatographic assay (CSS-UCNPs-dICA) combined with multiple-impurity adsorption purification (MIA) pretreatment was designed for the simultaneous and rapid detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) and fumonisin B<sub>1</sub> (FB<sub>1</sub>) in pet food. Capitalizing on the superior luminescent properties of the CSS-UCNPs, the assay exhibited high sensitivity with limits of detection (LODs) of 0.025 ng/mL for AFB<sub>1</sub> and 0.25 ng/mL for FB<sub>1</sub>. This represents a 20-fold sensitivity enhancement compared to colloidal gold-based immunochromatography. In spiking experiments, the assay achieved recoveries ranging from 97.69% − 104.66% for AFB<sub>1</sub> and 97.68%–105.26% for FB<sub>1</sub> in pet food samples. The generated results were consistent with data from liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses of naturally contaminated pet foods, confirming the superior practicality, rapidness, and simplicity. Thus, the developed CSS-UCNPs-dICA strip is a promising tool for the rapid, on-site, and large-scale screening of AFB<sub>1</sub> and FB<sub>1</sub> co-contamination in pet food.</p> Graphical Abstract <p></p>

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Core-shell-shell up-conversion nanoparticles-based fluorescence immunochromatographic assay for simultaneous quantitative detection of aflatoxin B1 and fumonisin B1 in pet food

  • 期刊论文

  • 发表日期：

    2026年3月3日

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  • Xinyao Zheng,
  • Xiuling Zhao,
  • Yuhuan Zhang,
  • Jianfeng Wang,
  • Yingying Zhong,
  • Junli Zhu

摘要

A novel dual-channel core-shell-shell up-conversion nanoparticle-based fluorescence immunochromatographic assay (CSS-UCNPs-dICA) combined with multiple-impurity adsorption purification (MIA) pretreatment was designed for the simultaneous and rapid detection of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) in pet food. Capitalizing on the superior luminescent properties of the CSS-UCNPs, the assay exhibited high sensitivity with limits of detection (LODs) of 0.025 ng/mL for AFB1 and 0.25 ng/mL for FB1. This represents a 20-fold sensitivity enhancement compared to colloidal gold-based immunochromatography. In spiking experiments, the assay achieved recoveries ranging from 97.69% − 104.66% for AFB1 and 97.68%–105.26% for FB1 in pet food samples. The generated results were consistent with data from liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses of naturally contaminated pet foods, confirming the superior practicality, rapidness, and simplicity. Thus, the developed CSS-UCNPs-dICA strip is a promising tool for the rapid, on-site, and large-scale screening of AFB1 and FB1 co-contamination in pet food.

Graphical Abstract