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<p>An amino-functionalized copper-based metal–organic framework (Cu–NH₂–BDC) is reported that integrates intrinsic fluorescence with versatile catalytic functions, including oxidase (OXD), peroxidase (POD), and laccase (LAC) activities. Engineering amino group and the central metal node enables Cu–NH₂–BDC to overcome the intrinsic pH limitations of most nanozymes, achieving its highest catalytic efficiency at physiological pH (7.4). Remarkably, Cu–NH₂–BDC exhibits substrate-switchable catalytic behavior, in which the substrate itself directs the dominant enzymatic pathway. This unique mechanism allows precise regulation of substrate-specific catalytic signaling while minimizing interference among multiple catalytic modes. Leveraging these properties, we established a triple-mode biosensing platform capable of highly sensitive and selective detection of acetylcholinesterase (AChE; LOD = 0.001 mU/mL), dopamine (DA; LOD = 0.048&#xa0;µg/mL), and ascorbic acid (AA; LOD = 0.6 µM) under physiological conditions. This work introduces a novel substrate-engineering strategy for modulating multi-colorimetric signal in nanozymes and provides a powerful and generalizable approach for developing intelligent multifunctional biosensors.</p> Graphical Abstract <p></p>

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One nanozyme, three functions: substrate-switchable Cu–NH₂–BDC nanozyme for multiplexed biosensing in physiological environments

  • 期刊论文

  • 发表日期：

    2026年3月1日

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  • Zhiyuan Luo,
  • Yuwan Lu,
  • Ya Wang,
  • Runrun Yang,
  • Yixin Liu,
  • Guobin Xiong,
  • Lingyun Cheng,
  • Hailong Shi,
  • Yaling Yuan,
  • Siqi Li

摘要

An amino-functionalized copper-based metal–organic framework (Cu–NH₂–BDC) is reported that integrates intrinsic fluorescence with versatile catalytic functions, including oxidase (OXD), peroxidase (POD), and laccase (LAC) activities. Engineering amino group and the central metal node enables Cu–NH₂–BDC to overcome the intrinsic pH limitations of most nanozymes, achieving its highest catalytic efficiency at physiological pH (7.4). Remarkably, Cu–NH₂–BDC exhibits substrate-switchable catalytic behavior, in which the substrate itself directs the dominant enzymatic pathway. This unique mechanism allows precise regulation of substrate-specific catalytic signaling while minimizing interference among multiple catalytic modes. Leveraging these properties, we established a triple-mode biosensing platform capable of highly sensitive and selective detection of acetylcholinesterase (AChE; LOD = 0.001 mU/mL), dopamine (DA; LOD = 0.048 µg/mL), and ascorbic acid (AA; LOD = 0.6 µM) under physiological conditions. This work introduces a novel substrate-engineering strategy for modulating multi-colorimetric signal in nanozymes and provides a powerful and generalizable approach for developing intelligent multifunctional biosensors.

Graphical Abstract